{"created":"2023-05-15T15:00:08.706597+00:00","id":81622,"links":{},"metadata":{"_buckets":{"deposit":"cca7d571-e679-446b-a3f5-333e107c00cf"},"_deposit":{"created_by":1,"id":"81622","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"81622"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00081622","sets":["1"]},"author_link":["995464","995455","995459","995450","995456","995462","995454","995460","995461","995463","995457","995458","995453","995451","995452"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"4371","bibliographicPageStart":"4357","bibliographicVolumeNumber":"10 ","bibliographic_titles":[{"bibliographic_title":"American journal of cancer research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Carbon ion radiotherapy (CIRT) is more effective than conventional photon beam radiotherapy in treating osteosarcoma (OSA); however, the outcomes of CIRT alone are still unsatisfactory. In this study, we aimed to investigate whether  acts as a radiosensitizer for CIRT. The OSA cell lines U2OS and KHOS were treated with carbon ion beam alone, γ-ray irradiation alone, or in combination with an  mimic. OSA cell death as well as invasive and migratory abilities were analyzed through viability, colony formation, Transwell, and apoptosis assays.  expression was downregulated in OSA tissues compared to that in normal tissues and was associated with metastasis and relapse in patients with OSA. Further,  was found to directly target the transcription factor Sp1 and suppress the activation of the phosphatase and tensin homolog (PTEN)-AKT pathway. Conversely, Sp1 was found to attenuate the inhibitory effects of  in OSA cells. When used in combination with  mimic, carbon ion beam markedly inhibited invasion, migration, and proliferation of OSA cells and promoted apoptosis by inhibiting AKT phosphorylation in a Sp1/PTEN-mediated manner. Taken together,  mimic improved the radiosensitivity of OSA cells via the PTEN-AKT-Sp1 signaling pathway, presenting a novel strategy for the development of carbon ion beam combination therapy.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"e-Century Publishing Corporation"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"33415004","subitem_relation_type_select":"PMID"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ajcr.us/files/ajcr0119320.pdf","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2156-6976","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ho Kim, Eun"}],"nameIdentifiers":[{"nameIdentifier":"995450","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yub Kim, Jeong"}],"nameIdentifiers":[{"nameIdentifier":"995451","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mi-Sook, Kim"}],"nameIdentifiers":[{"nameIdentifier":"995452","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Vares, Guillaume"}],"nameIdentifiers":[{"nameIdentifier":"995453","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohno, Tatsuya"}],"nameIdentifiers":[{"nameIdentifier":"995454","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takahashi, Akihisa"}],"nameIdentifiers":[{"nameIdentifier":"995455","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Uzawa, Akiko"}],"nameIdentifiers":[{"nameIdentifier":"995456","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Seung-Jun, Seo"}],"nameIdentifiers":[{"nameIdentifier":"995457","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sai, Sei"}],"nameIdentifiers":[{"nameIdentifier":"995458","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ho Kim, Eun","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"995459","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Vares, Guillaume","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"995460","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tatsuya, Ohno","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"995461","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Akihisa, Takahashi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"995462","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Akiko, Uzawa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"995463","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sei, Sai","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"995464","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Molecular mechanisms underlying the enhancement of carbon ion beam radiosensitivity of osteosarcoma cells by miR-29b","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Molecular mechanisms underlying the enhancement of carbon ion beam radiosensitivity of osteosarcoma cells by miR-29b"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-01-11"},"publish_date":"2021-01-11","publish_status":"0","recid":"81622","relation_version_is_last":true,"title":["Molecular mechanisms underlying the enhancement of carbon ion beam radiosensitivity of osteosarcoma cells by miR-29b"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:01:43.327406+00:00"}