@article{oai:repo.qst.go.jp:00081479,
 author = {Yoshimoto, Yuya and Sasaki, Yasushi and Murata, Kazutoshi and Shin-ei, Noda and Miyasaka, Yuhei and Hamamoto, Junko and Furuya, Mio and Hirato, Junko and Suzuki, Yoshiyuki and Ohno, Tatsuya and Tokino, Takashi and Oike, Takahiro and Nakano, Takashi and Tatsuya, Ohno and Takashi, Nakano},
 issue = {2},
 journal = {Gynecologic Oncology},
 month = {Dec},
 note = {Objective
To elucidate tumor mutation profiles associated with outcomes of uterine cervical cancer (UCC) patients treated with definitive radiotherapy.

Methods
Ninety-eight patients with newly diagnosed and pathologically confirmed UCC (82 squamous cell carcinomas, 12 adenocarcinomas, and four adenosquamous carcinomas) who were treated with definitive radiotherapy were analyzed. DNA was extracted from pre-treatment tumor biopsy specimens. The exons of 409 cancer-related genes were sequenced using a next-generation sequencer. Genetic mutations were identified and analyzed for correlations with clinical outcome.

Results
Recurrent mutations were observed in PIK3CA (35.7%), ARID1A (25.5%), NOTCH1 (19.4%), FGFR3 (16.3%), FBXW7 (19.4%), TP53 (13.3%), EP300 (12.2%), and FGFR4 (10.2%). The prevalence of mutations in FGFR family genes (i.e., FGFR1–4) was almost as high (24.5%) as that in PIK3CA and ARID1A, both of which are well-studied drivers of UCC. Fifty-five percent (21 of 38) of the identified FGFR mutations were located in the FGFR protein tyrosine kinase domain. Five-year progression-free survival (PFS) rates for FGFR mutation-positive patients (n = 24) were significantly worse than those for FGFR mutation-negative patients (n = 74) (43.9% vs. 68.5%, respectively; P = 0.010). Multivariate analysis identified FGFR mutations as significant predictors of worse 5 year PFS (P = 0.005), independent of clinicopathological variables.

Conclusions
FGFR mutations are associated with worse PFS in UCC patients treated with definitive radiotherapy. These results warrant further validation in prospective studies.},
 pages = {546--553},
 title = {Mutation profiling of uterine cervical cancer patients treated with definitive radiotherapy},
 volume = {159},
 year = {2020}
}