{"created":"2023-05-15T14:59:52.900797+00:00","id":81258,"links":{},"metadata":{"_buckets":{"deposit":"01b17c4f-2b72-412e-b357-3d432b61c3c7"},"_deposit":{"created_by":1,"id":"81258","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"81258"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00081258","sets":["1"]},"author_link":["1006047","1006046","1006040","1006037","1006041","1006038","1006044","1006045","1006043","1006042","1006039"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-12","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"12635","bibliographicPageStart":"12625","bibliographicVolumeNumber":"13","bibliographic_titles":[{"bibliographic_title":"OncoTargets and Therapy"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: The aim of this study was to investigate whether carbon-ion beam irradiation in combination with 5-fluorouracil (5-FU) is superior to carbon-ion beam irradiation alone in targeting colorectal cancer stem-like cells (CSCs).\nMaterials and Methods: Human colorectal cancer (CRC) cells, HCT116 and HT29, were treated with carbon-ion beam irradiation alone or in combination with 5-FU. Cell viability assay, colony and spheroid formation assay, apoptotic assay, and quantitative real-time PCR analysis of apoptosis- and autophagy-related gene expression were performed.\nResults: Carbon-ion beam irradiation dose-dependently decreased CRC cell viability and showed significantly enhanced cell killing effect when combined with 5-FU. Carbon-ion beam irradiation in combination with 5-FU significantly increased the percentage of apoptotic cells. The expression of some apoptotic and autophagy-related genes such as Bax, Bcl2,Beclin1 and ATG7 was significantly induced by carbon-ion beam irradiation alone and was further enhanced when the beam was combined with 5-FU. The spheroid forming capacity of CD133+ cell subpopulations was significantly inhibited by carbon-ion beam in combination with 5-FU. Histopathologically, the combination of carbon-ion beam irradiation and 5-FU destroyed more xenograft tumor cells, and resulted in increased necrosis, cavitation, and fibrosis, compared to carbon-ion beam irradiation alone.\nConclusion: In conclusion, carbon-ion beam treatment combined with 5-FU has the potential to kill CRC cells including CSCs by inducing increased apoptosis and autophagy.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Dove Medical Press"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.2147/OTT.S276035","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.dovepress.com/superior-effect-of-the-combination-of-carbon-ion-beam-irradiation-and--peer-reviewed-fulltext-article-OTT","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1178-6930","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sub Koom, Woong"}],"nameIdentifiers":[{"nameIdentifier":"1006037","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sai, Sei"}],"nameIdentifiers":[{"nameIdentifier":"1006038","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Masao"}],"nameIdentifiers":[{"nameIdentifier":"1006039","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujimori, Akira"}],"nameIdentifiers":[{"nameIdentifier":"1006040","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamada, Shigeru"}],"nameIdentifiers":[{"nameIdentifier":"1006041","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsujii, Hirohiko"}],"nameIdentifiers":[{"nameIdentifier":"1006042","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sei, Sai","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1006043","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Masao, Suzuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1006044","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Akira, Fujimori","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1006045","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shigeru, Yamada","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1006046","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirohiko, Tsujii","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1006047","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Superior Effect of the Combination of Carbon-Ion Beam Irradiation and 5-Fluorouracil on Colorectal  Cancer Stem Cells in vitro and in vivo","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Superior Effect of the Combination of Carbon-Ion Beam Irradiation and 5-Fluorouracil on Colorectal  Cancer Stem Cells in vitro and in vivo"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-12-10"},"publish_date":"2020-12-10","publish_status":"0","recid":"81258","relation_version_is_last":true,"title":["Superior Effect of the Combination of Carbon-Ion Beam Irradiation and 5-Fluorouracil on Colorectal  Cancer Stem Cells in vitro and in vivo"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:21:46.327595+00:00"}