{"created":"2023-05-15T14:59:44.178008+00:00","id":81054,"links":{},"metadata":{"_buckets":{"deposit":"b87bc435-4203-497c-9fa2-de22e75a6dec"},"_deposit":{"created_by":1,"id":"81054","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"81054"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00081054","sets":["1"]},"author_link":["1008179","1008181","1008182","1008183","1008178","1008180"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-11","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"1223","bibliographicPageStart":"1213","bibliographicVolumeNumber":"68","bibliographic_titles":[{"bibliographic_title":"Genetic Resources and Crop Evolution"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Ion-beam irradiation serves as a powerful tool of mutagenesis that engineers novel traits in a wide range of plants. A previous study indicated that coincidental changes of multiple traits are frequently induced by ion-beam irradiation in soybean, a paleopolyploid plant whose genome comprises a large number of duplicated genes. Here we analyzed the levels of isoflavones, the major secondary compounds accumulated in soybean seeds, in a population of mutants having chlorophyll deficiency of various degrees obtained by the mutagenesis. Significant reduction in the levels of four isoflavones, namely, daidzin, genistin, and their malonylglucosides, was detected in 4–9 plant lines among 28 lines analyzed. The average levels of chlorophyll in the leaf tissues and isoflavones in seeds are correlated with each other. In selected mutant lines, the levels of isoflavones were lower than the wild-type plants throughout seed development. Gene expression profiles indicated that the mRNA levels of multiple genes involved in the isoflavone synthesis pathway reduced during seed development, which suggests that the observed reduction in the levels of isoflavones may be attributable to gene expression changes. Along with the possibility that multiple and independent mutations that reduce the levels of chlorophyll and isoflavones occurred simultaneously, these observations suggest the presence of an unrecognized physiological link between the synthesis and/or accumulation of these compounds in soybean.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s10722-020-01061-9","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://link.springer.com/article/10.1007/s10722-020-01061-9","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0925-9864","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shiroshita, Yui"}],"nameIdentifiers":[{"nameIdentifier":"1008178","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hase, Yoshihiro"}],"nameIdentifiers":[{"nameIdentifier":"1008179","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamada, Tetsuya"}],"nameIdentifiers":[{"nameIdentifier":"1008180","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Abe, Jun"}],"nameIdentifiers":[{"nameIdentifier":"1008181","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kanazawa, Akira"}],"nameIdentifiers":[{"nameIdentifier":"1008182","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshihiro, Hase","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1008183","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of chlorophyll-deficient soybean [Glycine max (L.) Merr.] mutants obtained by ion-beam irradiation reveals concomitant reduction in isoflavone levels","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of chlorophyll-deficient soybean [Glycine max (L.) Merr.] mutants obtained by ion-beam irradiation reveals concomitant reduction in isoflavone levels"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-11-02"},"publish_date":"2020-11-02","publish_status":"0","recid":"81054","relation_version_is_last":true,"title":["Characterization of chlorophyll-deficient soybean [Glycine max (L.) Merr.] mutants obtained by ion-beam irradiation reveals concomitant reduction in isoflavone levels"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:13:13.478904+00:00"}