{"created":"2023-05-15T14:59:37.696695+00:00","id":80906,"links":{},"metadata":{"_buckets":{"deposit":"9b5e0d3f-5708-4885-b4ed-c657a162ee01"},"_deposit":{"created_by":1,"id":"80906","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"80906"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00080906","sets":["10:29"]},"author_link":["899285","899280","899286","899279","899287","899281","899282","899289","899284","899288","899290","899283"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2020-03-23","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Aminopeptidase N (APN) is an enzyme which preferentially cleaves peptides with neutral residues at N-terminus. This peptidase has attracted many interests as an important biomarker of cancers because of its relationship with malignancy, metastasis and angiogenesis. However, APN-activity detection in deep areas has not been achieved although several kinds of fluorescent and luminescent probes have been developed to date1. \nNuclear magnetic resonance (NMR) is a powerful technique to monitor molecules in a body because this technique has advantages in high tissue penetration and low invasiveness, allowing a molecular detection at deep area. However, the sensitivity of NMR is severely low for in vivo application. To overcome this problem, dynamic nuclear polarization (DNP) has emerged. NMR signals are dramatically enhanced by using this method.\nPreviously, a DNP-NMR molecular probe for detection of APN-activity has been reported by our group2. This probe was designed in points of view of APN-selectivity and spin-lattice relaxation time (T1) related with hyperpolarized lifetime. By using this probe, APN-activity was successfully detected in experiments in vitro. However, this probe has not been applicable in vivo. \nIn this study, we have developed a practical molecular probe for DNP-NMR to detect APN-activity in vivo through rational molecular design based on the previous probe. It is demonstrated that this probe has many advantages for in vivo application and enables detection of APN-activity in xenograft tumors bearing mice. \n\n1) C. L. Schreiber, B. D. Smith, Contrast Media & Molecular Imaging 2018, 2018, 5315172. 2) R. Hata, H. Nonaka, Y. Takakusagi, K. Ichikawa, S. Sando, Angew. Chem., Int. Ed. 2016, 55, 1765.\n","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"日本化学会第100 回春季年会 2020","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Saito, Yutaro"}],"nameIdentifiers":[{"nameIdentifier":"899279","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kondo, Yohei"}],"nameIdentifiers":[{"nameIdentifier":"899280","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishida, Ryo"}],"nameIdentifiers":[{"nameIdentifier":"899281","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Seki, Tomohiro"}],"nameIdentifiers":[{"nameIdentifier":"899282","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tamura, Iori"}],"nameIdentifiers":[{"nameIdentifier":"899283","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yatabe, Hiroyuki"}],"nameIdentifiers":[{"nameIdentifier":"899284","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takakusagi, Yoichi"}],"nameIdentifiers":[{"nameIdentifier":"899285","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamamoto, Kazutoshi"}],"nameIdentifiers":[{"nameIdentifier":"899286","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nonaka, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"899287","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"C Krishna, Murali"}],"nameIdentifiers":[{"nameIdentifier":"899288","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sando, Shinsuke"}],"nameIdentifiers":[{"nameIdentifier":"899289","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takakusagi, Yoichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"899290","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Development of a Hyperpolarized Molecular Probe for in vivo Detection of Aminopeptidase N Activity","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development of a Hyperpolarized Molecular Probe for in vivo Detection of Aminopeptidase N Activity"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-11-06"},"publish_date":"2020-11-06","publish_status":"0","recid":"80906","relation_version_is_last":true,"title":["Development of a Hyperpolarized Molecular Probe for in vivo Detection of Aminopeptidase N Activity"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:20:01.888350+00:00"}