@article{oai:repo.qst.go.jp:00080823,
 author = {Miyagi, Atsuko and Takuya Saimaru and Harigai, Nozomi and Ono, Yutaka and Hase, Yoshihiro and Maki, Kawai-Yamada and Yutaka, Ono and Yoshihiro, Hase},
 issue = {9},
 journal = {Metabolomics},
 month = {Sep},
 note = {Rice leaves and stems, which can be used as rice straw for livestock feed, accumulate soluble oxalate. The oxalate content often reaches 5% of the dry weight leaves. Excess uptake of oxalate-rich plants causes mineral deficiencies in vertebrates, so it is important to reduce the oxalate content in rice leaves to produce high-quality rice straw. However, the mechanism of oxalate accumulation in rice has remained unknown. In this study, we performed metabolome analysis of rice M 2 population generated by ion-beam irradiation using CE-MS to understand metabolic networks relating oxalate accumulation in rice. The result showed wide variation of oxalate contents in M 2 plants compared with those of control plants. Multivariate analyses of metabolome dataset revealed that oxalate accumulation was strongly related with anionic compounds such as 2OG and succinate. For low-oxalate plants, four patterns of metabolic alterations affected oxalate contents in the M 2 leaves were observed. In M 3 plants, we found putative low-oxalate line obtained from low-oxalate M 2 mutant. These findings would lead to produce the low-oxalate rice and to understand the oxalate synthesis in plants.},
 title = {Metabolome analysis of rice leaves to obtain low-oxalate strain from ion beam-mutagenised population},
 volume = {16},
 year = {2020}
}