@misc{oai:repo.qst.go.jp:00080822,
 author = {Miyagi, Atsuko and Saimaru, Takuya and Harigai, Nozomi and Ono, Yutaka and Hase, Yoshihiro and Ono, Yutaka and Hase, Yoshihiro},
 month = {Jul},
 note = {Rice straw obtained from rice leaves and stems can be used as a livestock feed. Rice leaves accumulate soluble oxalate, which easily binds to cationic ions such as calcium, iron, and magnesium ions, then becomes insoluble. It is an important agricultural topic to reduce oxalate content in crops because excess uptake of oxalate-rich plants causes mineral racking or urinary syndrome for human and livestock, although it is a useful molecule for plants to defense themselves from predators, to reduce aluminum toxicity in the acidic soil, to regulate calcium content in their body, and to provide hydrogen peroxide which acts as a signal molecule during aging or wounding. Three pathways (isocitrate, glycolate and ascorbate pathways) have been reported for oxalate synthesis in plants. However, it has been unknown which pathway contributes the oxalate accumulation in rice leaves. For reducing oxalate content in rice leaves and understanding oxalate synthesis mechanism, we first grew rice mutagenized M2 population (Koshihikari) generated from seeds irradiated by carbon ion beams and measured oxalate contents in their leaves. The oxalate measurement using CE-MS showed that there was no difference between means of oxalate contents in the population of M2 plants and those in the control plants, but wider variation of oxalate contents was observed in M2 plants compared with control plants. Metabolomic analyses of oxalate and other metabolite in the M2 plants showed that organic acids such as 2OG and succinate were decreased in the low-oxalate rice plants, although amino acids except for alanine, glutamate, and aspartate were increased. These suggested that oxalate accumulation in rice leaves would be affected by carbon flow via isocitrate pathway, and that reduction of oxalate contents might lead to increase of amino acids contents., Plant Biology 2020},
 title = {Alteration of oxalate content in rice leaves obtained from ion beam-irradiated plants},
 year = {2020}
}