{"created":"2023-05-15T14:59:21.185405+00:00","id":80524,"links":{},"metadata":{"_buckets":{"deposit":"f77b9e31-6eb6-43b6-82ef-9b3cf2c8dc52"},"_deposit":{"created_by":1,"id":"80524","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"80524"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00080524","sets":["10:28"]},"author_link":["891430","891429","891424","891426","891428","891431","891425","891427"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2020-09-17","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A small DNA binding protein to target desired sequences have the potential to become a scaffold of molecular tools such as genome-editing enzymes. We previously showed two engrailed homeodomains (EHDs) connected with a linker recognizes a target sequence twice as long as a single EHD in cells only when arginine 53 in each EHD in the tandem protein is mutated to alanine ((EHD[R53A])2). In this study, we determined the crystal structure of the (EHD[R53A])2-DNA complex. Most importantly, it shows the base-specific interactions necessary for the affinity and/or specificity of the wild-type EHD are preserved in (EHD[R53A])2. The mechanism of the specific recognition will be discussed based on the structure and cellular assays.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第58回日本生物物理学会年会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sunami, Tomoko"}],"nameIdentifiers":[{"nameIdentifier":"891424","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirano, Yuu"}],"nameIdentifiers":[{"nameIdentifier":"891425","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tamada, Taro"}],"nameIdentifiers":[{"nameIdentifier":"891426","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kono, Hidetoshi"}],"nameIdentifiers":[{"nameIdentifier":"891427","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sunami, Tomoko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"891428","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirano, Yuu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"891429","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tamada, Taro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"891430","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kono, Hidetoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"891431","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Structural basis for an array of engrailed homeodomains toward the development of genome-editing enzymes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Structural basis for an array of engrailed homeodomains toward the development of genome-editing enzymes"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-06-25"},"publish_date":"2020-06-25","publish_status":"0","recid":"80524","relation_version_is_last":true,"title":["Structural basis for an array of engrailed homeodomains toward the development of genome-editing enzymes"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:28:36.137576+00:00"}