{"created":"2023-05-15T14:59:19.038977+00:00","id":80478,"links":{},"metadata":{"_buckets":{"deposit":"bc46e9d7-1023-439c-bca4-4159de61f0eb"},"_deposit":{"created_by":1,"id":"80478","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"80478"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00080478","sets":["1"]},"author_link":["907789","907790","907792","907793","907788","907787","907794","907795","907796","907791","907786","907785","907797"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"2386","bibliographicPageStart":"2371","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"American journal of cancer research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To investigate whether carbon-ion beam alone, or in combination with lapatinib, has a beneficial effect in targeting HER2-positive breast cancer stem-like cells (CSCs) compared to that of X-rays, human breast CSCs derived from BT474 and SKBR3 cell lines were treated with a carbon-ion beam or X-rays irradiation alone or in combination with lapatinib, and then cell viability, spheroid formation assays, apoptotic analyses, gene expression analysis of related genes, and -H2AX foci  were performed. Spheroid formation assays confirmed that ESA+/CD24- cells have CSC properties compared to ESA-/CD24+ cells. CSCs were more highly enriched after X-ray irradiation combined with lapatinib, whereas carbon-ion beam combined with lapatinib significantly decreased the proportion of CSCs. Carbon-ion beam combined with lapatinib significantly suppressed spheroid formation compared to X-rays combined with lapatinib or carbon ion beam alone. Cell cycle analysis showed that carbon ion beam combined with lapatinib predominantly enhanced sub-G1 and G2/M arrested population compared to that of carbon-ion beam, X-ray treatments alone. Carbon-ion beam combined with lapatinib significantly enhanced apoptosis and carbon-ion beam alone dose-dependently increased autophagy-related expression of Beclin1 and in combination with lapatinib greatly enhanced ATG7 expression at protein levels. In addition, a large-sized H2AX foci in CSCs were induced by carbon ion beam combined with lapatinib treatment in CSCs compared to cells receiving X-rays or carbon-ion beam alone. Altogether, combination of carbon-ion beam irradiation and lapatinib has a high potential to kill HER2-positive breast CSCs, causing severe irreparable DNA damage, enhanced autophagy, and apoptosis.","subitem_description_type":"Abstract"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"32905515","subitem_relation_type_select":"PMID"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://ajcr.us/AJCR_V10N8.html","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2156-6976","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sai, Sei"}],"nameIdentifiers":[{"nameIdentifier":"907785","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ho Kim, Eun"}],"nameIdentifiers":[{"nameIdentifier":"907786","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Vares, Guillaume"}],"nameIdentifiers":[{"nameIdentifier":"907787","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Masao"}],"nameIdentifiers":[{"nameIdentifier":"907788","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yu, Dong"}],"nameIdentifiers":[{"nameIdentifier":"907789","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Horimoto, Yoshiya"}],"nameIdentifiers":[{"nameIdentifier":"907790","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hayashi, Mitsuhiro"}],"nameIdentifiers":[{"nameIdentifier":"907791","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sai, Sei","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"907792","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ho Kim, Eun","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"907793","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Vares, Guillaume","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"907794","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Masao","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"907795","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yu, Dong","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"907796","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Horimoto, Yoshiya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"907797","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Combination of carbon-ion beam and dual tyrosine kinase inhibitor, lapatinib, effectively destroys HER2 positive breast cancer stem-like cells.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Combination of carbon-ion beam and dual tyrosine kinase inhibitor, lapatinib, effectively destroys HER2 positive breast cancer stem-like cells."}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-09-11"},"publish_date":"2020-09-11","publish_status":"0","recid":"80478","relation_version_is_last":true,"title":["Combination of carbon-ion beam and dual tyrosine kinase inhibitor, lapatinib, effectively destroys HER2 positive breast cancer stem-like cells."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:07:40.943114+00:00"}