{"created":"2023-05-15T14:59:18.400763+00:00","id":80464,"links":{},"metadata":{"_buckets":{"deposit":"69dbea47-4a50-4b4f-b582-1bd9cf9a0469"},"_deposit":{"created_by":1,"id":"80464","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"80464"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00080464","sets":["1"]},"author_link":["936567","936565","936563","936561","936566","936564","936562"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"647","bibliographicPageStart":"639","bibliographicVolumeNumber":"61","bibliographic_titles":[{"bibliographic_title":"Journal of Radiation Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To investigate the involvement of the non-homologous end joining (NHEJ) pathway in plant mutagenesis by ionizing radiation, we conducted a genome-wide characterization of the mutations induced by gamma rays in NHEJ-deficient Arabidopsis mutants (AtKu70−/− and AtLig4−/−). Although both mutants were more sensitive to gamma rays than the wild-type control, the AtKu70−/− mutant was slightly more sensitive than the AtLig4−/− mutant. Single-base substitutions (SBSs) were the predominant mutations in the wild-type control, whereas deletions (≥ 2 bp) and complex-type mutations [i.e., more than two SBSs or short insertion and deletions (InDels) separated by fewer than 10 bp] were frequently induced in the mutants. Single-base deletions were the most frequent deletions in the wild-type control, whereas the most common deletions in the mutants were 11–30 bp. The apparent microhomology at the rejoined sites of deletions peaked at 2 bp in the wild-type control, but was 3–4 bp in the mutants. This suggests the involvement of alternative end joining and single-strand annealing pathways involving increased microhomology for rejoining DNA ends. Complex-type mutations comprising short InDels were frequently detected in the mutants, but not in the wild-type control. Accordingly, NHEJ is more precise than the backup pathways, and is the main pathway for rejoining the broken DNA ends induced by ionizing radiation in plants.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/jrr/rraa059","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://academic.oup.com/jrr/article/61/5/639/5881841","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1349-9157","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yan, DU"}],"nameIdentifiers":[{"nameIdentifier":"936561","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hase, Yoshihiro"}],"nameIdentifiers":[{"nameIdentifier":"936562","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sato, Katsuya"}],"nameIdentifiers":[{"nameIdentifier":"936563","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shikazono, Naoya"}],"nameIdentifiers":[{"nameIdentifier":"936564","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshihiro, Hase","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"936565","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Katsuya, Sato","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"936566","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Naoya, Shikazono","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"936567","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of gamma irradiation-induced mutations in Arabidopsis mutants deficient in non-homologous end joining","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of gamma irradiation-induced mutations in Arabidopsis mutants deficient in non-homologous end joining"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-04-13"},"publish_date":"2020-04-13","publish_status":"0","recid":"80464","relation_version_is_last":true,"title":["Characterization of gamma irradiation-induced mutations in Arabidopsis mutants deficient in non-homologous end joining"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:30:53.851654+00:00"}