@article{oai:repo.qst.go.jp:00080464,
 author = {Yan, DU and Hase, Yoshihiro and Sato, Katsuya and Shikazono, Naoya and Yoshihiro, Hase and Katsuya, Sato and Naoya, Shikazono},
 issue = {5},
 journal = {Journal of Radiation Research},
 month = {Sep},
 note = {To investigate the involvement of the non-homologous end joining (NHEJ) pathway in plant mutagenesis by ionizing radiation, we conducted a genome-wide characterization of the mutations induced by gamma rays in NHEJ-deficient Arabidopsis mutants (AtKu70−/− and AtLig4−/−). Although both mutants were more sensitive to gamma rays than the wild-type control, the AtKu70−/− mutant was slightly more sensitive than the AtLig4−/− mutant. Single-base substitutions (SBSs) were the predominant mutations in the wild-type control, whereas deletions (≥ 2 bp) and complex-type mutations [i.e., more than two SBSs or short insertion and deletions (InDels) separated by fewer than 10 bp] were frequently induced in the mutants. Single-base deletions were the most frequent deletions in the wild-type control, whereas the most common deletions in the mutants were 11–30 bp. The apparent microhomology at the rejoined sites of deletions peaked at 2 bp in the wild-type control, but was 3–4 bp in the mutants. This suggests the involvement of alternative end joining and single-strand annealing pathways involving increased microhomology for rejoining DNA ends. Complex-type mutations comprising short InDels were frequently detected in the mutants, but not in the wild-type control. Accordingly, NHEJ is more precise than the backup pathways, and is the main pathway for rejoining the broken DNA ends induced by ionizing radiation in plants.},
 pages = {639--647},
 title = {Characterization of gamma irradiation-induced mutations in Arabidopsis mutants deficient in non-homologous end joining},
 volume = {61},
 year = {2020}
}