{"created":"2023-05-15T14:59:12.609695+00:00","id":80336,"links":{},"metadata":{"_buckets":{"deposit":"8177816e-5668-4c40-b8b5-61d368edc134"},"_deposit":{"created_by":1,"id":"80336","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"80336"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00080336","sets":["10:29"]},"author_link":["882316","882318","882324","882315","882320","882321","882322","882323","882317","882325","882319"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2020-11-26","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"DNA-タンパク質クロスリンク損傷 (DPCs) は、放射線や環境変異原外的並びに代謝過程など内的要因で生じるDNA損傷である。その損傷修復の初期過程で必要となるクロスリンク蛋白の分解に、メタロプロテアーゼの一種であるSPRTNが関与すると考えられている。しかし、DPCs形成蛋白のうちSPRTNがどの蛋白に関わるかについて、細胞レベルでの詳細な解析はない。そこで、ヒトリンパ細胞由来のTK6でSPRTNを欠く細胞を作成し、DPCs誘発薬剤に対する感受性及びDPCs除去能について、野生株と比較した。SPRTN欠損細胞は、様々な蛋白にてDPCsを生じるフォルムアルデヒド(FA)には感受性を示さなかったが、DNAメチルトランスフェラーゼ1(Dnmt1)を特異的にDNAへトラップするアザデオキシシチジン(azadC)に対しては強い感受性を示した。さらにazadC及びFAで生成した種々のDPCs量に対し、野生株とSPRTN欠損細胞の修復効率を比較したところ、SPRTN欠損細胞ではDnmt1-DPCsが顕著に残存していた。以上のことから、DPCs形成蛋白のうちDnmt1の分解に対し、SPRTNが特異的に関与していることがヒト細胞において示唆された。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"日本環境変異原学会第49回大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"中野, 敏彰"}],"nameIdentifiers":[{"nameIdentifier":"882315","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"笹沼, 博之"}],"nameIdentifiers":[{"nameIdentifier":"882316","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"津田, 雅貴"}],"nameIdentifiers":[{"nameIdentifier":"882317","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"廣田, 耕志"}],"nameIdentifiers":[{"nameIdentifier":"882318","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"赤松, 憲"}],"nameIdentifiers":[{"nameIdentifier":"882319","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"川西, 優喜"}],"nameIdentifiers":[{"nameIdentifier":"882320","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"武田, 俊一"}],"nameIdentifiers":[{"nameIdentifier":"882321","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"井出, 博"}],"nameIdentifiers":[{"nameIdentifier":"882322","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田野, 恵三"}],"nameIdentifiers":[{"nameIdentifier":"882323","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakano, Toshiaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"882324","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Akamatsu, Ken","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"882325","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Participation of SPRTN in the repair of azadC-induced DNA-protein cross-links in human TK6 cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Participation of SPRTN in the repair of azadC-induced DNA-protein cross-links in human TK6 cells"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-08-18"},"publish_date":"2020-08-18","publish_status":"0","recid":"80336","relation_version_is_last":true,"title":["Participation of SPRTN in the repair of azadC-induced DNA-protein cross-links in human TK6 cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:39:25.419246+00:00"}