{"created":"2023-05-15T14:59:09.873866+00:00","id":80274,"links":{},"metadata":{"_buckets":{"deposit":"8b2f8af7-0741-416c-856d-3a37ba095594"},"_deposit":{"created_by":1,"id":"80274","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"80274"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00080274","sets":["1"]},"author_link":["885208","885211","885207","885214","885216","885210","885209","885213","885215","885212"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-08","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"37","bibliographicPageStart":"33","bibliographicVolumeNumber":"84-85","bibliographic_titles":[{"bibliographic_title":"Nuclear Medicine and Biology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Technetium-99m-labeled mercaptoacetyltriglycine ([99mTc]MAG3) is widely used for evaluation of transplanted kidneys, diagnosis of tubular necrosis, and scintigraphic studies of tubular function. [99mTc]MAG3 is a substrate for organic anion transporter (OAT)1 and OAT3 on the basolateral membrane side for renal secretion. We investigated the transport mechanism and affinity of [99mTc]MAG3 on the apical membrane of renal proximal tubule cells for renal secretion. Adenosine triphosphate-binding cassette (ABC) transporters for renal secretion of [99mTc]MAG3 were examined using ABC transporter vesicles expressing multiple drug resistance 1 (MDR1), breast cancer resistance protein (BCRP), multidrug resistance-associated protein (MRP)2, and MRP4. MK-571, a MRP inhibitor, was applied to measure the Km and Vmax of MRP2 and MRP4 in a vesicle transport assay. Single photon emission computed tomography (SPECT) was performed in normal rats and MRP2-deficient Eisai hyperbilirubinuria rats (EHBR) using [99mTc]MAG3 with and without MK-571. [99mTc]MAG3 uptake in adenosine triphosphate was significantly higher than that in adenosine monophosphate in vesicles that highly expressed MRP2 and MRP4. The affinity of [99mTc]MAG3 for MRP4 was higher than that for MRP2. Renal secretion via MRP2 and MRP4 was identified by comparing normal and EHBR rats with and without MK-571 on SPECT. [99mTc]MAG3 is transported via MRP2 and MRP4 on the apical membrane of renal proximal tubule cells. The affinity of MRP4 is higher than that of MRP2.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.nucmedbio.2020.01.002","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/pii/S0969805119305426","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0969-8051","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kobayashi, Masato"}],"nameIdentifiers":[{"nameIdentifier":"885207","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishi, Kodai"}],"nameIdentifiers":[{"nameIdentifier":"885208","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mizutani, Asuka"}],"nameIdentifiers":[{"nameIdentifier":"885209","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okudaira, Hiroyuki"}],"nameIdentifiers":[{"nameIdentifier":"885210","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakanishi, Takeo"}],"nameIdentifiers":[{"nameIdentifier":"885211","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shikano, Naoto"}],"nameIdentifiers":[{"nameIdentifier":"885212","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishii, Ryuichi"}],"nameIdentifiers":[{"nameIdentifier":"885213","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tamai, Ikumi"}],"nameIdentifiers":[{"nameIdentifier":"885214","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawai, Keiichi"}],"nameIdentifiers":[{"nameIdentifier":"885215","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishii, Ryuichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"885216","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Transport mechanism and affinity of [99mTc]Tc-mercaptoacetyltriglycine ([99mTc]MAG3) on the apical membrane of renal proximal tubule cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Transport mechanism and affinity of [99mTc]Tc-mercaptoacetyltriglycine ([99mTc]MAG3) on the apical membrane of renal proximal tubule cells"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-08-04"},"publish_date":"2020-08-04","publish_status":"0","recid":"80274","relation_version_is_last":true,"title":["Transport mechanism and affinity of [99mTc]Tc-mercaptoacetyltriglycine ([99mTc]MAG3) on the apical membrane of renal proximal tubule cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:36:04.365727+00:00"}