{"created":"2023-05-15T14:59:02.018303+00:00","id":80093,"links":{},"metadata":{"_buckets":{"deposit":"590bf1cf-b5d8-4ba3-ade8-ba1d62edc3d0"},"_deposit":{"created_by":1,"id":"80093","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"80093"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00080093","sets":["1"]},"author_link":["1024683","1024690","1024693","1024688","1024685","1024686","1024687","1024689","1024684","1024682","1024692","1024691"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"130","bibliographicPageStart":"123","bibliographicVolumeNumber":"68","bibliographic_titles":[{"bibliographic_title":"Journal of Clinical Biochemistry and Nutrition"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Relatively young (4-week-old) selenium deficient (SeD) mice, which lack the activity of selenium-dependent glutathione peroxidase (GSH-Px) isomers, were prepared using torula yeast-based SeD diet.\nMice were fed the torula yeast-based SeD diet and ultra-pure water. Several different timings for starting the SeD diet were assessed. The weekly time course of liver comprehensive GSH-Px activity after weaning was monitored. Protein expression levels of GPx1 and 4 in the liver were measured by Western blot analysis. Gene expression levels of GPx1, 2, 3, 4, and 7 in the liver were measured by quantitative real-time PCR. Apoptotic activity of thymocytes after H2O2 exposure was compared. Thirty-day survival rates after whole-body X-ray irradiation were estimated.\nPre-birth or right-after-birth starting of the SeD diet in dams was unable to lead to creation of SeD mice due to neonatal death. This suggests that Se is necessary for normal birth and healthy growing of mouse pups. Starting the mother on the SeD diet from 2 weeks after giving birth (SeD-trial-2w group) resulted in a usable SeD mouse model. The liver GSH-Px activity of the SeD-trial-2w group was almost none from 4 week olds, but the mice survived for more than 63 weeks. Protein and gene expression of GPx1 was suppressed in the SeD-trial-2w group, but that of GPx4 was not. The thymocytes of the SeD-trial-2w group were sensitive to H2O2-induced apoptosis. The SeD-trial-2w group was sensitive to whole-body X-ray irradiation compared with control mice. The SeD-trial-2w model may be a useful animal model for H2O2/hydroperoxide-induced oxidative stress.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本酸化ストレス学会"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"33879963","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3164/jcbn.20-36","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.jstage.jst.go.jp/article/jcbn/68/2/68_20-36/_pdf/-char/en","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0912-0009","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ueno, Megumi"}],"nameIdentifiers":[{"nameIdentifier":"1024682","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimokawa, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"1024683","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Emiko, Sekine-Suzuki"}],"nameIdentifiers":[{"nameIdentifier":"1024684","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nyui, Minako"}],"nameIdentifiers":[{"nameIdentifier":"1024685","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakanishi, Ikuo"}],"nameIdentifiers":[{"nameIdentifier":"1024686","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ken-ichiro, Matsumoto"}],"nameIdentifiers":[{"nameIdentifier":"1024687","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Megumi, Ueno","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1024688","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takashi, Shimokawa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1024689","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Emiko, Sekine","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1024690","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Minako, Nyui","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1024691","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ikuo, Nakanishi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1024692","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kenichiro, Matsumoto","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1024693","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-03-28"}],"displaytype":"detail","filename":"8efd77f6877b8af4fa212ba2bda79155.pdf","filesize":[{"value":"641.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Ueno.M.2020.J.Clin.Biochem.Nutr.68(2).123-130.Se-deficinet.mouse.pdf","url":"https://repo.qst.go.jp/record/80093/files/8efd77f6877b8af4fa212ba2bda79155.pdf"},"version_id":"590e3b33-9afd-4952-9c53-cdc679f2264e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Preparation of an experimental mouse model lacking selenium-dependent glutathione peroxidase activities by feeding a selenium-deficient diet","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Preparation of an experimental mouse model lacking selenium-dependent glutathione peroxidase activities by feeding a selenium-deficient diet"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-11-05"},"publish_date":"2019-11-05","publish_status":"0","recid":"80093","relation_version_is_last":true,"title":["Preparation of an experimental mouse model lacking selenium-dependent glutathione peroxidase activities by feeding a selenium-deficient diet"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T18:05:24.580746+00:00"}