@inproceedings{oai:repo.qst.go.jp:00079954,
 author = {Ohsawa, Daisuke and Kobayashi, Alisa and Konishi, Teruaki and Ohsawa, Daisuke and Kobayashi, Alisa and Konishi, Teruaki},
 book = {Proceedings of the 8th Asia Pacific Symposium on Radiation Chemistry (APSRC2020)},
 month = {May},
 note = {Advanced ion microbeam technology is attracting ever increasing interest for radiobiological studies, from the viewpoint that the microbeam can deliver precise absorbed dose to pre-selected individual cells in vitro and analyze their response at the single cell level. Until now, we have built the Single Particle Irradiation system to Cell, SPICE in the National Institutes for Quantum and Radiological Science and Technology(QST) and have extensively performed various experiments on microdosimetric radiobiology such as radiation-induced bystander effect. The SPICE can deliver a focused vertical microbeam of 3.4 MeV protons to either or both of a targeted cell nucleus and cytoplasm with a high spatial resolution of approximately 2 μm and with the highest irradiation speed of 400 cells/min. Such advantages of the SPICE have motivated further investigations on how DNA damage complexity within the cell nucleus does affect the response in the targeted cell.
Previously, we reported an accurate and systematic investigation of the SPICE microbeam profile using fluorescent nuclear track detectors (FNTDs). In the present study, we focused on the localized dose distribution in the targeted cell nuclei irradiated with a defined number of protons and its correlation with intensity and area of DNA double strand breaks (DSBs) damage and repair region, visualized by immunostaining against g-H2AX and 53BP1. Kinetics of induced g-H2AX and 53BP1 fluorescent spots was analyzed by Z(optical axis)-stacks of confocal laser scanning microscopic images for the irradiated cell nuclei and the details will be presented in this conference.},
 title = {Spatiotemporal dynamics of DNA damage and repair regions from focused proton tracks in human cells after SPICE microbeam irradiation},
 year = {2020}
}