{"created":"2023-05-15T14:58:27.333052+00:00","id":79306,"links":{},"metadata":{"_buckets":{"deposit":"521a760f-e483-4361-b4fa-da8b8879a07a"},"_deposit":{"created_by":1,"id":"79306","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"79306"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00079306","sets":["7"]},"author_link":["850110","850108","850112","850111","850109","850113","850107","850106"],"item_10004_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"88","bibliographicVolumeNumber":"QST-M-23","bibliographic_titles":[{"bibliographic_title":"QST Takasaki Annual Report 2018"}]}]},"item_10004_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Actinomycetes, filamentous Gram-positive bacteria, are a major source of bioactive natural products which can be drug candidates. We are studying about the bacterial-bacterial interaction induced secondary metabolism by filamentous actinomycetes e.g. Streptomyces species. Mycolic acid-containing bacteria, Tsukamurella pulmonis TP-B0596 (Tp) possess ability to induce production of secondary metabolites in a range of filamentous actinomycetes, which are not produced in their single culture. Object of this study is to elucidate the gene(s) which are involved in the response for activation of secondary metabolism within filamentous actinomycetes. Elucidation of the mechanism may lead for development of versatile genetic tools to discover novel bioactive natural products. S. lividans TK23 and S.s coelicolor JCM4020 were observed to produce red pigment compound (RED) in response to direct cell-cell contact interaction of Tp. We investigated the gene(s) responsible for the activation of RED which was induced by Tp using S. coelicolor JCM4020 as a model to explore the molecular basis of the response mechanism. We employed carbon ion beams (12C5+, 220 MeV), accelerated by the AVF cyclotron at TIARA, to induce mutagenesis on spores to generate mutant library of S. coelicolor. We screened the RED production deficient mutants of S. coelicolor by mixing them with Tp on agar plates. Using red / white color phenotype of the colonies as indication,  we obtained 59 mutants which were deficient in induced RED production by Tp, but were normal in their apparent growth and morphological development. We re-sequenced the genome of 16 mutants using MiSeq and identified mutational points existed in CDS. ","subitem_description_type":"Abstract"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Asamizu, Shunpei"}],"nameIdentifiers":[{"nameIdentifier":"850106","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishizuka, Takumi"}],"nameIdentifiers":[{"nameIdentifier":"850107","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yanagisawa, Masaomi"}],"nameIdentifiers":[{"nameIdentifier":"850108","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sato, Katsuya"}],"nameIdentifiers":[{"nameIdentifier":"850109","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ono, Yutaka"}],"nameIdentifiers":[{"nameIdentifier":"850110","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Onaka, Hiroyasu"}],"nameIdentifiers":[{"nameIdentifier":"850111","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sato, Katsuya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"850112","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ono, Yutaka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"850113","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Identification of Genes Responsible for the Contact-dependent RED Response in Streptomyces coelicolor","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification of Genes Responsible for the Contact-dependent RED Response in Streptomyces coelicolor"}]},"item_type_id":"10004","owner":"1","path":["7"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-03-11"},"publish_date":"2020-03-11","publish_status":"0","recid":"79306","relation_version_is_last":true,"title":["Identification of Genes Responsible for the Contact-dependent RED Response in Streptomyces coelicolor"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T22:30:42.272478+00:00"}