@article{oai:repo.qst.go.jp:00078896,
 author = {Funayama, Tomoo and Sakashita, Tetsuya and Suzuki, Michiyo and Yokota, Yuichiro and Miyawaki, Nobumasa and Kashiwagi, Hirotsugu and Satoh, Takahiro and Kurashima, Satoshi and Tomo, Funayama and Tetsuya, Sakashita and Michiyo, Suzuki and Yuuichiro, Yokota and Nobumasa, Miyawaki and Hirotsugu, Kashiwagi and Takahiro, Satoh and Satoshi, Kurashima},
 journal = {Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms},
 month = {Jan},
 note = {For use in medical and life science research, a device and sample preparation methods were developed to irradiate cultured cells and individual animals with a focused microbeam of cyclotron-accelerated heavy-ions. The new irradiation device positions both sample and microscope with micrometer precision. The beam size is smaller than 3 μm when the distance between the beam exit from vacuum to the irradiation device is less than 500 μm for 13.0 MeV/n 20Ne ions. This beam size is sufficiently small to irradiate intracellular targets in cultured cells. A beam size smaller than 10 μm was obtained for 12C ions of 26.7 MeV/n at a distance of 2 mm from the beam exit, which enabled irradiation of specific cells in a small model animal. Methods to prepare cultured cells and the nematode Caenorhabditis elegans  for irradiation using the new device are reported, and the results of the first irradiation experiments presented.},
 pages = {101--109},
 title = {An irradiation device for biological targets using focused microbeams of cyclotron-accelerated heavy ion},
 volume = {465},
 year = {2020}
}