{"created":"2023-05-15T14:57:51.119086+00:00","id":78473,"links":{},"metadata":{"_buckets":{"deposit":"12d9c3a7-5e47-4b41-b387-d1cd3106e210"},"_deposit":{"created_by":1,"id":"78473","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"78473"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00078473","sets":["1"]},"author_link":["1001192","1001184","1001191","1001189","1001185","1001190","1001187","1001193","1001188","1001186"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"496","bibliographicPageStart":"492","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"The Journal of Physical Chemistry Letters"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Neutron crystallography has been used to elucidate the protonation states for the enhanced green fluorescent protein, which has revolutionized the imaging technologies. The structure has a deprotonated hydroxyl group in the fluorescent chromophore. Also, the protonation states of His148 and Thr203, as well as the orientation of a critical water molecule in direct contact with the chromophore, could be determined. The results demonstrate that the deprotonated hydroxyl group in the chromophore and the nitrogen atom ND1 in His148 are charged negatively and positively, respectively, forming an ion pair. The position of the two deuterium atoms in the critical water molecule appears to be displaced slightly toward the acceptor oxygen atoms according to their omit maps. This displacement implies the formation of an intriguing electrostatic potential realized inside the protein. Our findings provide new insights into strategy for future protein design with developments for quantum chemical calculations.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"ACS Publications"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"31880458","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1021/acs.jpclett.9b03252","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://pubs.acs.org/doi/10.1021/acs.jpclett.9b03252","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1948-7185","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shibazaki, Chie"}],"nameIdentifiers":[{"nameIdentifier":"1001184","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimizu, Rumi"}],"nameIdentifiers":[{"nameIdentifier":"1001185","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kagotani, Yuji"}],"nameIdentifiers":[{"nameIdentifier":"1001186","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ostermann, Andreas"}],"nameIdentifiers":[{"nameIdentifier":"1001187","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"E. Schrader, Tobias"}],"nameIdentifiers":[{"nameIdentifier":"1001188","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Adachi, Motoyasu"}],"nameIdentifiers":[{"nameIdentifier":"1001189","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Chie, Shibazaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1001190","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Rumi, Shimizu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1001191","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yuji, Kagotani","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1001192","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Motoyasu, Adachi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1001193","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Direct Observation of the Protonation States in the Mutant Green Fluorescent Protein","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Direct Observation of the Protonation States in the Mutant Green Fluorescent Protein"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-12-17"},"publish_date":"2019-12-17","publish_status":"0","recid":"78473","relation_version_is_last":true,"title":["Direct Observation of the Protonation States in the Mutant Green Fluorescent Protein"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:38:35.920903+00:00"}