{"created":"2023-05-15T14:57:46.645902+00:00","id":78401,"links":{},"metadata":{"_buckets":{"deposit":"168d5b80-99e0-4a19-b9d8-7d3a977b63ff"},"_deposit":{"created_by":1,"id":"78401","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"78401"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00078401","sets":["1"]},"author_link":["1001229","1001227","1001221","1001225","1001226","1001231","1001222","1001228","1001230","1001224","1001223"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"84","bibliographicPageStart":"73","bibliographicVolumeNumber":"76","bibliographic_titles":[{"bibliographic_title":"Acta Crystallographica section D"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"AbstractThioredoxin (TRX) is an important antioxidant against oxidative stress. TRX from Halobacterium salinarum NRC-1 (HsTRX-A), which has the highest acidic residue content ([Asp + Glu] / [Arg + Lys + His] = 9.0) among known TRXs, was chosen to elucidate a catalytic mechanism and evolutionary characteristics associated with haloadaptation. X-ray crystallographic analysis revealed that the main chain structure of HsTRX-A is similar to those of homologous TRXs; e.g., root-mean-square deviations for Cα atoms were <2.3 Å for the extant archaeal TRXs and <1.5 Å for the resurrected Precambrian TRXs. The density of negative charges at the molecular surface (0.0035 Å-2) and the structural flexibility of the long N-terminal coil (Arg1–Pro16) of HsTRX-A were higher than those of homologous TRXs and should improve solubility and haloadaptivity. Circular dichroism measurement indicated that the structure of HsTRX-A is highly flexible and that a high salt concentration (~4 M NaCl) is required for structural stabilization. A unique water network was located near the active-site residues (Cys47 and Cys50) in HsTRX-A, which may enhance the proton transfer required for reduction of substrates under a high-salt environment. A structural inspection of TRXs revealed that helix α1 in TRXs elongated during evolution from LACA-TRX (the last archaeal common ancestor TRX) to extant archaeal TRXs, including HsTRX-A. These observations offer insights into molecular evolution for haloadaptation and potential applications in halophilic protein-related biotechnology.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"International Union of Crystallography"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"31909745","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1107/S2059798319015894","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://scripts.iucr.org/cgi-bin/paper?S2059798319015894","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2059-7983","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Arai, Shigeki"}],"nameIdentifiers":[{"nameIdentifier":"1001221","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shibazaki, Chie"}],"nameIdentifiers":[{"nameIdentifier":"1001222","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimizu, Rumi"}],"nameIdentifiers":[{"nameIdentifier":"1001223","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Adachi, Motoyasu"}],"nameIdentifiers":[{"nameIdentifier":"1001224","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishibashi, Matsujiro"}],"nameIdentifiers":[{"nameIdentifier":"1001225","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tokunaga, Hiroko"}],"nameIdentifiers":[{"nameIdentifier":"1001226","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tokunaga, Masao"}],"nameIdentifiers":[{"nameIdentifier":"1001227","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shigeki, Arai","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1001228","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Chie, Shibazaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1001229","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Rumi, Shimizu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1001230","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Motoyasu, Adachi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1001231","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Catalytic mechanism and evolutional characteristics of thioredoxin from Halobacterium salinarum NRC-1","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Catalytic mechanism and evolutional characteristics of thioredoxin from Halobacterium salinarum NRC-1"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-08-05"},"publish_date":"2019-08-05","publish_status":"0","recid":"78401","relation_version_is_last":true,"title":["Catalytic mechanism and evolutional characteristics of thioredoxin from Halobacterium salinarum NRC-1"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:38:26.227099+00:00"}