@misc{oai:repo.qst.go.jp:00077750,
 author = {Konishi, Teruaki and Ohsawa, Daisuke and Kobayashi, Alisa and Oikawa, Masakazu and Konishi, Teruaki and Ohsawa, Daisuke and Kobayashi, Alisa and Oikawa, Masakazu},
 month = {Nov},
 note = {Advanced microbeam irradiation system made possible to deliver a defined amount of radiation on a single cell with a few micrometer resolution. Our microbeam, the Single-Particle Irradiation system to CEll (SPICE) provides a 3.4 MeV proton microbeam focused with a quadrupole magnetic lens on an upward vertical beam line (1,2). SPICE is the only proton microbeam in Japan that can target nucleus, cytoplasm, or both with high throughput, high targeting accuracy along with promising stability. In this presentation, the state of the art features of SPICE will be introduced along with our recent results from single cell analysis of microbeam targeted cells on defensive signalling through intra- and inter-cellular response against radiation, such as cytoplasmic damage response (3,4), and cell-to-cell communication between human normal and carcinoma cells (5-7). 

1. T. Konishi, et al., J Radiat Res 54(2013) 736-747. 
2. D. Ohsawa, et al., Nucl Instrum Methods Phys. Res B 453 (2019) 9-14.
3. J. Wang and T. Konishi, Cancer Sci, 110 (2019) 686-696.
4. T. Konishi, et al., J. Radiat Cancer Res 9 (2018)183-189.
5. A. Kobayashi, et al., Radiat Prot Dosim 183 (2019) 142-146.
6. N. Autsavapromporn, et al., Radiat Res 191 (2019) 211-216.
7. A. Kobayashi, et al., Mutat Res-Fund Mol M 803-805 (2017) 1-8., 日本顕微鏡学会第６２回シンポジウム},
 title = {Advanced microbeam irradiation system for single cell analysis of defensive cellular response against radiation},
 year = {2019}
}