{"created":"2023-05-15T14:57:14.494001+00:00","id":77677,"links":{},"metadata":{"_buckets":{"deposit":"7d1643b3-6f75-413c-b408-acac8b744d6e"},"_deposit":{"created_by":1,"id":"77677","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"77677"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00077677","sets":["1"]},"author_link":["998644","998658","998646","998655","998659","998654","998657","998650","998651","998649","998645","998656","998647","998653","998648","998652"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"39","bibliographicPageEnd":"6646","bibliographicPageStart":"6630","bibliographicVolumeNumber":"38","bibliographic_titles":[{"bibliographic_title":"Oncogene"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Tumor-treating fields (TTFs) - a type of electromagnetic field-based therapy using low-intensity electrical fields - has recently been characterized as a potential anticancer therapy for glioblastoma multiforme (GBM). However, the molecular mechanisms involved remain poorly understood. Our results show that the activation of autophagy contributes to the TTF-induced anti-GBM activity in vitro or in vivo and GBM patient stem cells or primary in vivo culture systems. TTF-treatment upregulated several autophagy-related genes (~2-fold) and induced cytomorphological changes. TTF-induced autophagy in GBM was associated with decreased Akt2 expression, not Akt1 or Akt3, via the mTOR/p70S6K pathway. An Affymetrix GeneChip miRNA 4.0 Array analysis revealed that TTFs altered the expression of many microRNAs (miRNAs). TTF-induced autophagy upregulated miR-29b, which subsequently suppressed the Akt signaling pathway. A luciferase reporter assay confirmed that TTFs induced miR-29b to target Akt2, negatively affecting Akt2 expression thereby triggering autophagy. TTF-induced autophagy suppressed tumor growth in GBM mouse models subjected to TTFs as determined by positron emission tomography and computed tomography (PET-CT). GBM patient stem cells and a primary in vivo culture system with high Akt2 levels also showed TTF-induced inhibition. Taken together, our results identified autophagy as a critical cell death pathway triggered by TTFs in GBM and indicate that TTF is a potential treatment option for GBM.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nature Publishing Group"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"31375748","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/s41388-019-0882-7","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0950-9232","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ho Kim, Eun"}],"nameIdentifiers":[{"nameIdentifier":"998644","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Jo, Yunhui"}],"nameIdentifiers":[{"nameIdentifier":"998645","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sai, Sei"}],"nameIdentifiers":[{"nameIdentifier":"998646","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mung-Jin, Park"}],"nameIdentifiers":[{"nameIdentifier":"998647","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Jeong-Yub, Kim"}],"nameIdentifiers":[{"nameIdentifier":"998648","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Su Kim, Jin"}],"nameIdentifiers":[{"nameIdentifier":"998649","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yeon-Joo, Lee"}],"nameIdentifiers":[{"nameIdentifier":"998650","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Jae-Min, Cho"}],"nameIdentifiers":[{"nameIdentifier":"998651","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Seo-Young, Kwak"}],"nameIdentifiers":[{"nameIdentifier":"998652","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Jeong-Hwa, Baek"}],"nameIdentifiers":[{"nameIdentifier":"998653","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kyoung Jeong, Youn"}],"nameIdentifiers":[{"nameIdentifier":"998654","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Jie-Young, Song"}],"nameIdentifiers":[{"nameIdentifier":"998655","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoon, Myonggeun"}],"nameIdentifiers":[{"nameIdentifier":"998656","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sang-Gu, Hwang"}],"nameIdentifiers":[{"nameIdentifier":"998657","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ho Kim, Eun","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"998658","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sei, Sai","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"998659","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Tumor-treating fields induce autophagy by blocking the Akt2/miR29b axis in glioblastoma cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Tumor-treating fields induce autophagy by blocking the Akt2/miR29b axis in glioblastoma cells"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-11-28"},"publish_date":"2019-11-28","publish_status":"0","recid":"77677","relation_version_is_last":true,"title":["Tumor-treating fields induce autophagy by blocking the Akt2/miR29b axis in glioblastoma cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:48:13.519854+00:00"}