{"created":"2023-05-15T14:57:09.513268+00:00","id":77564,"links":{},"metadata":{"_buckets":{"deposit":"0824c9cf-8ce8-4b25-b96a-a2521d74ae42"},"_deposit":{"created_by":1,"id":"77564","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"77564"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00077564","sets":["1"]},"author_link":["914777","914769","914774","914773","914776","914775","914768","914772","914771","914778","914770"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-09","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"13413","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"Scientific Reports"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We examined whether [131I]6-β-iodomethyl-19-norcholesterol (NP-59), a cholesterol analog, can be used to measure function of hepatic drug transporters. Hepatic uptake of NP-59 with and without rifampicin was evaluated using HEK293 cells expressing solute carrier transporters. The stability of NP-59 was evaluated using mouse blood, bile, and liver, and human liver S9. Adenosine triphosphate-binding cassette (ABC) transporters for bile excretion were examined using hepatic ABC transporter vesicles expressing multidrug resistance protein 1, multidrug resistance-associated protein (MRP)1-4, breast cancer resistance protein (BCRP), or bile salt export pump with and without MK-571 and Ko143. Single photon emission computed tomography (SPECT) was performed in normal mice injected with NP-59 in the presence or absence of Ko143. Uptake of NP-59 into HEK293 cells expressing organic anion transporting polypeptide (OATP)1B1 and OATP1B3 was significantly higher than that into mock cells and was inhibited by rifampicin. NP-59 was minimally metabolized in mouse blood, bile, and liver, and human liver S9 after 120 min of incubation. In vesicles, NP-59 was transported by MRP1 and BCRP. Excretion of NP-59 into bile via BCRP was observed in normal mice with and without Ko143 in the biological distribution and SPECT imaging. NP-59 can be used to visualize and measure the hepatic function of OATP1B1, OATP1B3, and BCRP.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/s41598-019-50049-8","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.nature.com/articles/s41598-019-50049-8","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2045-2322","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kobayashi, Masato"}],"nameIdentifiers":[{"nameIdentifier":"914768","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishi, Kodai"}],"nameIdentifiers":[{"nameIdentifier":"914769","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mizutani, Asuka"}],"nameIdentifiers":[{"nameIdentifier":"914770","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsuzumi Hokama"}],"nameIdentifiers":[{"nameIdentifier":"914771","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsue, Miki"}],"nameIdentifiers":[{"nameIdentifier":"914772","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"TetsuyaTsujikawa"}],"nameIdentifiers":[{"nameIdentifier":"914773","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakanishi, Takeo"}],"nameIdentifiers":[{"nameIdentifier":"914774","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishii, Ryuichi"}],"nameIdentifiers":[{"nameIdentifier":"914775","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"IkumiTamai"}],"nameIdentifiers":[{"nameIdentifier":"914776","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawai, Keiichi"}],"nameIdentifiers":[{"nameIdentifier":"914777","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishii, Ryuichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"914778","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Imaging of hepatic drug transporters with [131I]6-β-iodomethyl-19-norcholesterol","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Imaging of hepatic drug transporters with [131I]6-β-iodomethyl-19-norcholesterol"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-11-21"},"publish_date":"2019-11-21","publish_status":"0","recid":"77564","relation_version_is_last":true,"title":["Imaging of hepatic drug transporters with [131I]6-β-iodomethyl-19-norcholesterol"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:59:44.710915+00:00"}