@misc{oai:repo.qst.go.jp:00077505,
 author = {Ihara, Tomokazu and Noguchi, Miho and Yokoya, Akinari and Ihara, Tomokazu and Noguchi, Miho and Yokoya, Akinari},
 month = {Nov},
 note = {Autophagy of ionizing irradiated cells is well known
to correlated with induction of their senescence, and
might also be involved in senescence or carcinogenesis of
other cells surrounding them. Those evidences suggest
that intracellular environment in senescent cells would
be different from that of normal cells. To reveal the
intracellular autophagy activities in human fibroblast
cells after 20Gy X-ray exposure and to understand the
relationship between cell senescence and autophagy,
we analyzed the expression level of p16 and p21, which
are involved in senescence, and LC3 and pp62, which
play a role in autophagy, by western blotting of extract
from irradiated cells. The expression level of p16 and
p21 gradually increased after irradiation. Although the
expression level of LC3 also increased until a few days
after exposure, pp62 decreased rapidly. It is inferred
that pp62 is necessary for selective autophagy, but be
decomposed after the autophagy. For these processes,
it might contribute to intracellular homeostasis. These
results indicated that selective autophagy could be
activated immediately after irradiation, and then,
non-selective autophagy could be activated and cause
secretion of certain factors inducing late effects, such as
carcinogenesis of surrounding cells. For further study,
application of a novel technique using nano-diamonds
will be discussed in terms of sensing physical parameters, such as temperature, in the senescent cells., 日本放射線影響学会第62回大会},
 title = {Changes of intracellular environment and autophagy activity during senescence induction after X-ray exposure.},
 year = {2019}
}