{"created":"2023-05-15T14:57:03.131411+00:00","id":77418,"links":{},"metadata":{"_buckets":{"deposit":"672402f4-1805-45ac-bf34-21defb76cc62"},"_deposit":{"created_by":1,"id":"77418","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"77418"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00077418","sets":["10:28"]},"author_link":["860331","860328","860330","860326","860327","860332","860329"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2019-11-14","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Whole cell irradiation inevitably causes various\ndamage in not only genomic DNA but also intracellular\norganelles. In order to separately examine repairability\nof DNA damage from damage responses of organelles,\nEGFP-expressing plasmid DNA (in TE buffer) was\nexposed to X-rays in vitro, and then transfected into \"\nNON-irradiated \" human breast cancer cells (MCF7). The\ndynamics of EGFP fluorescence of cells was observed\nby a live cell imaging technique to analyze DNA repair\nefficiency in the cells. The EGFP expression kinetics as\nDNA repair efficiency was analyzed for the cells transfected\nwith the plasmids exposed to several X-ray doses.\nThe similar experiments were also performed using\nplasmids treated with nicking or restriction enzyme to\nobtain positive control data of SSB or DSB induction,\nrespectively. Based on those positive control values,\ntheoretically expected efficiencies were also calculated\nassuming that strand breakage of plasmid DNA by irradiation\nfollowed Poisson distribution. The numbers of\ncells expressing EGFP in the experimental results were\nconsiderably fewer than the theoretical values, indicating\nthat the irradiated plasmids were less repaired in the\ncells. These results strongly suggest that X-irradiation\ncauses complex types of strand break termini rather\nthan those created by enzymatic treatments, presumably\nso called non-DSB type clustered DNA damage.\nThese particular types of radiation induced damage\nwould compromise DNA repair efficiency, consequently\ninduce down-regulation of EGFP expression.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"日本放射線影響学会第62回大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Obata, Yui"}],"nameIdentifiers":[{"nameIdentifier":"860326","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirasaki, Keishiro"}],"nameIdentifiers":[{"nameIdentifier":"860327","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yokoya, Akinari"}],"nameIdentifiers":[{"nameIdentifier":"860328","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Akimitsu, Nobuyoshi"}],"nameIdentifiers":[{"nameIdentifier":"860329","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Obata, Yui","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"860330","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirasaki, Keishiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"860331","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yokoya, Akinari","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"860332","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Live cell observation of EGFP expression of in vitro X-irradiated plasmid DNA in non-irradiated human cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Live cell observation of EGFP expression of in vitro X-irradiated plasmid DNA in non-irradiated human cells"},{"subitem_title":"非照射ヒト細胞におけるin vitro X 線照射プラスミドDNA の EGFP 発現のライブセル観察","subitem_title_language":"en"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-11-13"},"publish_date":"2019-11-13","publish_status":"0","recid":"77418","relation_version_is_last":true,"title":["Live cell observation of EGFP expression of in vitro X-irradiated plasmid DNA in non-irradiated human cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T22:10:58.266015+00:00"}