{"created":"2023-05-15T14:56:49.198454+00:00","id":77112,"links":{},"metadata":{"_buckets":{"deposit":"72680177-2339-4c14-832b-21362635c970"},"_deposit":{"created_by":1,"id":"77112","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"77112"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00077112","sets":["1"]},"author_link":["998584","998578","998580","998577","998579","998583","998581","998586","998585","998582"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-09","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"1078","bibliographicPageStart":"1065","bibliographicVolumeNumber":"19","bibliographic_titles":[{"bibliographic_title":"iScience"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The coiled-coil motif mediates subunit oligomerization and scaffolding and underlies several fundamental biologic processes. Prohibitins (PHBs), mitochondrial inner membrane proteins involved in mitochondrial homeostasis and signal transduction, are predicted to have a coiled-coil motif, but their structural features are poorly understood. Here we solved the crystal structure of the heptad repeat (HR) region of PHB2 at 1.7 A resolution, showing that it assembles into a dimeric, antiparallel coiled-coil with a unique negatively charged area essential for the PHB interactome in mitochondria. Disruption of the HR coiled-coil abolishes well-ordered PHB complexes and the mitochondrial tubular networks accompanying PHB-dependent signaling. Using a proximity-dependent biotin identification (BioID) technique in live cells, we mapped a number of mitochondrial intermembrane space proteins whose association with PHB2 relies on the HR coiled-coil region. Elucidation of the PHB complex structure in mitochondria provides insight into essential PHB interactomes required for mitochondrial dynamics as well as signal transduction.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Cell Press"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.isci.2019.08.056","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/pii/S2589004219303359?via%3Dihub","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2589-0042","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yoshinaka, Takahiro"}],"nameIdentifiers":[{"nameIdentifier":"998577","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kosako, Hidetaka"}],"nameIdentifiers":[{"nameIdentifier":"998578","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshizumi, Takuma"}],"nameIdentifiers":[{"nameIdentifier":"998579","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furukawa, Ryo"}],"nameIdentifiers":[{"nameIdentifier":"998580","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirano, Yuu"}],"nameIdentifiers":[{"nameIdentifier":"998581","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kuge, Osamu"}],"nameIdentifiers":[{"nameIdentifier":"998582","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tamada, Taro"}],"nameIdentifiers":[{"nameIdentifier":"998583","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Koshiba, Takumi"}],"nameIdentifiers":[{"nameIdentifier":"998584","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yuu, Hirano","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"998585","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Taro, Tamada","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"998586","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Structural Basis of Mitochondrial Scaffolds by Prohibitin Complexes: Insight into a Role of the Coiled-Coil Region","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Structural Basis of Mitochondrial Scaffolds by Prohibitin Complexes: Insight into a Role of the Coiled-Coil Region"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-09-17"},"publish_date":"2019-09-17","publish_status":"0","recid":"77112","relation_version_is_last":true,"title":["Structural Basis of Mitochondrial Scaffolds by Prohibitin Complexes: Insight into a Role of the Coiled-Coil Region"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:48:26.821106+00:00"}