@misc{oai:repo.qst.go.jp:00077053,
 author = {Sai, Sei and Suzuki, Masao and Vares, Guillaume and Horimoto, Yoshiya and 林, 光弘 and Sai, Sei and Suzuki, Masao and Vares, Guillaume and Horimoto, Yoshiya},
 month = {Sep},
 note = {In the present study, we attempt to investigate effects of carbon-ion beam alone, or in combination with lapatinib, a dual tyrosine kinase inhibitor, on targeting HER2-positive breast cancer stem-like cells (CSCs). Spheroid formation assays confirmed that ESA+/CD24- cells have CSC properties compared to ESA-/CD24+ cells in HER2-positive breast cancer cells. Carbon-ion beam combined with lapatinib significantly suppressed spheroid formation compared to X-rays combined with lapatinib or carbon ion beam alone. Carbon-ion beam combined with lapatinib greatly enhanced apoptosis and also induced autophagy-related expression of Beclin1 and LC3 compared to carbon-ion beam alone, X-rays alone, or X-rays combined with lapatinib. In addition, a large-sized gammaH2AX foci in CSCs were induced by carbon ion beam combined with lapatinib treatment in CSCs compared to cells receiving X-rays or carbon-ion beam alone. In conclusion, combination of carbon-ion beam irradiation and lapatinib has a high potential to kill HER2-positive breast CSCs, causing severe irreparable DNA damage, enhanced autophagy, and apoptosis., 第78回日本癌学会学術総会},
 title = {Effects of Carbon-ion Beam and Lapatinib Alone, or in Combination in HER2 Positive Breast Cancer Stem-like Cells},
 year = {2019}
}