@misc{oai:repo.qst.go.jp:00076954, author = {角南, 智子 and ルオ, ディ and 河野, 秀俊 and Sunami, Tomoko and Luo, Di and Kono, Hidetoshi}, month = {Sep}, note = {The first nucleosomes in the downstream of the transcription starting sites, +1 nucleosomes, have been found to play critical roles in genome organization. We have previously proposed that consecutive AA/TT dimer sequences in the entry side of +1 nucleosomes tend to unwrap more easily than those without AA/TT (Luo et al, NAR, 2018). To reveal sequence-dependent unwrapping in more detail, we conducted FRET measurement of reconstituted nucleosomes with sequences containing AA/TT repeat or containing the other dimer repeats. Our time-correlated single photon counting analysis showed that (1) the open state population is the most abundant in the nucleosomes with AT-rich sequences and (2) the open state is the most widely opened on the nucleosomes with long AA/TT repeat., 第57回日本生物物理学会年会}, title = {FRET study on sequence-dependent unwrapping of nucleosomal DNA}, year = {2019} }