{"created":"2023-05-15T14:56:24.720266+00:00","id":76623,"links":{},"metadata":{"_buckets":{"deposit":"786f41d1-4e62-4280-9865-68e77cfbe739"},"_deposit":{"created_by":1,"id":"76623","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"76623"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00076623","sets":["10:29"]},"author_link":["779295","779296"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2019-08-26","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose Last few decades, more than 11000 cancer patients were treated by carbon-ion radiotherapy using HIMAC, and achieved promising outcomes. Here we attempt to elucidate underlying molecular mechanisms of high curability treated carbon-ion beams alone or in combination with DNA damaging drugs or with tyrosine kinase inhibitors or with microRNA modulators from the point of view of targeting cancer stem cells (CSCs). \nMethods Human pancreatic, liver, breast, and mesothelioma CSCs sorted from each cell line were treated with carbon-ion beam, X-ray irradiation alone or in combination with gemcitabine/cisplatin or sorafenib/lapatinib or miRNA200c mimic, and then cell viability assay, colony, spheroid formation ability assay, DNA damage assay (H2AX foci), and real time PCR analysis, and xenograft tumor control analysis were performed.\nResults The relative biological effectiveness (RBE) values for the carbon-ion beams relative to X-rays at the 10% survival levels for CSCs were estimated as 2.1 to 2.4. The colony and spheroid formation capability of CSCs was significantly inhibited by carbon-ion beam combined with gemcitabine/cisplatin or sorafenib/lapatinib or with micro RNA 200c mimic, accomplished with irreparable complex H2AX foci formation. RT Profiler PCR Array analysis showed that apoptosis- and autophagy-related gene expression was significantly induced after carbon ion beam combined with gemcitabine/cisplatin or with sorafenib/lapatinib compared to carbon ion alone in pancreatic, liver, breast cancer and mesothelioma cells. Xenograft tumors from pancreatic, liver, breast cancer and mesothelioma cells were effectively disrupted by 25 Gy of carbon-ion beam combined with 50 mg/kg gemcitabine for pancreatic xenograft tumors or 15 Gy of carbon-ion beam combined with 5 mg/kg cisplatin for breast and mesothelioma xenograft tumors. \nConclusions Carbon-ion beam has high potential to kill various CSCs, and in combination with DNA damaging drugs or with tyrosine kinase inhibitors or with microRNA modulators can enhance its effects.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"16th International Congress of Radiation Research","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sai, Sei"}],"nameIdentifiers":[{"nameIdentifier":"779295","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sai, Sei","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"779296","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"New Insight into Molecular Mechanisms of Carbon-Ion Radiobiology: Targeting Cancer Stem Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"New Insight into Molecular Mechanisms of Carbon-Ion Radiobiology: Targeting Cancer Stem Cells"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-09-02"},"publish_date":"2019-09-02","publish_status":"0","recid":"76623","relation_version_is_last":true,"title":["New Insight into Molecular Mechanisms of Carbon-Ion Radiobiology: Targeting Cancer Stem Cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:17:51.424930+00:00"}