@article{oai:repo.qst.go.jp:00075924, author = {尹, 永根 and 石井, 里美 and 鈴井, 伸郎 and 井倉, 将人(農研機構) and 栗田, 圭輔 and 三好, 悠太 and 長澤, 尚胤 and 田口, 光正 and 河地, 有木 and Yin, Yonggen and Satomi, Ishii and Nobuo, Suzui and Keisuke, Kurita and Yuta, Miyoshi and Naotsugu, Nagasawa and Mitsumasa, Taguchi and Naoki, Kawachi}, journal = {Applied Radiation and Isotopes}, month = {May}, note = {Accurate analysis of N fixation in leguminous crops requires determination of N utilization within an intact plant; however, most approaches require tissue disassembly. We developed a simple and rapid technique to generate high-purity and high-yield [13N]N2 gas and obtained real-time images of N fixation in an intact soybean plant. The purification efficiency was ~81.6% after decay correction. Our method provides accurate signals of N fixation and allows free changes to the tracer gas composition to suit different experimental designs.}, pages = {7--12}, title = {On-line rapid purification of [13N]N2 gas for visualization of nitrogen fixation and translocation in nodulated soybean}, volume = {151}, year = {2019} }