{"created":"2023-05-15T14:55:47.013624+00:00","id":75744,"links":{},"metadata":{"_buckets":{"deposit":"fed0f510-a187-4019-abbc-f60d4e1b0cbb"},"_deposit":{"created_by":1,"id":"75744","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"75744"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00075744","sets":["1"]},"author_link":["841134","841139","841131","841133","841138","841137","841142","841141","841143","841140","841135","841132","841144","841136"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"876","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"Nature Communications"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Serine hydroxymethyltransferase (SHMT) is an enzyme that catalyzes the reaction that converts serine to glycine. It plays an important role in one-carbon metabolism. Recently, SHMT has been shown to be associated with various diseases. Therefore, SHMT has attracted attention as a biomarker and drug target. However, the development of molecular probes responsive to SHMT has not yet been realized. This is because SHMT catalyzes an essential yet simple reaction; thus, the substrates that can be accepted into the active site of SHMT are limited. Here, we focus on the SHMT-catalyzed retro-aldol reaction rather than the canonical serine–glycine conversion and succeed in developing fluorescent and 19F NMR molecular probes. Taking advantage of the facile and direct detection of SHMT, the devel- oped fluorescent probe is used in the high-throughput screening for human SHMT inhibitors, and two hit compounds are obtained.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nature.com"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"30787298","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/s41467-019-08833-7","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.nature.com/articles/s41467-019-08833-7","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2041-1723","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nonaka, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"841131","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakanishi, Yuki"}],"nameIdentifiers":[{"nameIdentifier":"841132","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kuno, Satoshi"}],"nameIdentifiers":[{"nameIdentifier":"841133","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ota, Tomoki"}],"nameIdentifiers":[{"nameIdentifier":"841134","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mochidome, Kentaro"}],"nameIdentifiers":[{"nameIdentifier":"841135","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saito, Yutaro"}],"nameIdentifiers":[{"nameIdentifier":"841136","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sugihara, Fuminori"}],"nameIdentifiers":[{"nameIdentifier":"841137","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takakusagi, Yoichi"}],"nameIdentifiers":[{"nameIdentifier":"841138","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Aoki, Ichio"}],"nameIdentifiers":[{"nameIdentifier":"841139","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nagatoishi, Satoru"}],"nameIdentifiers":[{"nameIdentifier":"841140","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsumoto, Kouhei"}],"nameIdentifiers":[{"nameIdentifier":"841141","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sando, Shinsuke"}],"nameIdentifiers":[{"nameIdentifier":"841142","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takakusagi, Yoichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"841143","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Aoki, Ichio","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"841144","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Design strategy for serine hydroxymethyltransferase probes based on retro-aldol-type reaction","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Design strategy for serine hydroxymethyltransferase probes based on retro-aldol-type reaction"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-04-26"},"publish_date":"2019-04-26","publish_status":"0","recid":"75744","relation_version_is_last":true,"title":["Design strategy for serine hydroxymethyltransferase probes based on retro-aldol-type reaction"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T22:44:07.049279+00:00"}