{"created":"2023-05-15T14:55:15.095213+00:00","id":75094,"links":{},"metadata":{"_buckets":{"deposit":"4e812a1b-39fb-4d84-9074-e6e51957b796"},"_deposit":{"created_by":1,"id":"75094","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"75094"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00075094","sets":["1"]},"author_link":["822504","822502","822499","822503","822508","822500","822505","822501","822507","822498","822506"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-04","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"234","bibliographicPageStart":"228","bibliographicVolumeNumber":"222","bibliographic_titles":[{"bibliographic_title":"Life Sciences"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Aims\nIn comparison with a low linear energy transfer (LET) radiation, a high-LET radiation induces more complex DNA damage. This study wonders whether radiation-induced bystander effect (RIBE) is dependent of LET.\n\nMaterials and methods\nChinese hamster ovary CHO-9 cells and its subline EM-C11 cells (SSB repair deficient) and XR-C1 cells (DSB repair deficient) were irradiated by γ-rays, α-particles, or carbon ions with different LETs of 13, 30 and 70 keV/μm. Cell proliferation, cell death, DNA damage, cell cycle distribution and some protein expressions were measured with the cell counting kit-8 (CCK-8), colony formation, micronuclei (MN), flow cytometry and western blot, respectively.\n\nKey findings\nA series of cell responses were induced by these radiations in a LET-dependent manner, including proliferation inhibition, cell death, MN induction, G2/M phase arrest and the expression of γH2AX protein. These cell injuries were also depended on DNA repair capacity, and XR-C1 cells were the most sensitive to each radiation. Furthermore, when the cells were treated with the conditioned medium (CM) collected from irradiated CHO-9 cells, the MN induction and cell death response in the bystander cells of EM-C11 or XR-C1 increased along with LET of irradiation, and the bystander damage was easier to be induced in EM-C11 and XR-C1 cells than that in CHO-9 cells.\n\nSignificance\nBoth cellular DNA repair capacity and the LET value of radiation could deeply influence damage extents of not only the irradiated cells but also the bystander cells.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Inc."}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"30858123","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.lfs.2019.03.013","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/abs/pii/S0024320519301717?via%3Dihub","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0024-3205","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tu, Wenzhi"}],"nameIdentifiers":[{"nameIdentifier":"822498","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Dong, Chen"}],"nameIdentifiers":[{"nameIdentifier":"822499","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fu, Jiamei"}],"nameIdentifiers":[{"nameIdentifier":"822500","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Pan, Yan"}],"nameIdentifiers":[{"nameIdentifier":"822501","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kobayashi, Alisa"}],"nameIdentifiers":[{"nameIdentifier":"822502","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furusawa, Yoshiya"}],"nameIdentifiers":[{"nameIdentifier":"822503","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Konishi, Teruaki"}],"nameIdentifiers":[{"nameIdentifier":"822504","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shao, Chunlin"}],"nameIdentifiers":[{"nameIdentifier":"822505","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kobayashi, Alisa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"822506","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furusawa, Yoshiya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"822507","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Konishi, Teruaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"822508","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Both irradiated and bystander effects link with DNA repair capacity and the linear energy transfer","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Both irradiated and bystander effects link with DNA repair capacity and the linear energy transfer"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-03-12"},"publish_date":"2019-03-12","publish_status":"0","recid":"75094","relation_version_is_last":true,"title":["Both irradiated and bystander effects link with DNA repair capacity and the linear energy transfer"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:16:16.352592+00:00"}