@article{oai:repo.qst.go.jp:00075094,
 author = {Tu, Wenzhi and Dong, Chen and Fu, Jiamei and Pan, Yan and Kobayashi, Alisa and Furusawa, Yoshiya and Konishi, Teruaki and Shao, Chunlin and Kobayashi, Alisa and Furusawa, Yoshiya and Konishi, Teruaki},
 journal = {Life Sciences},
 month = {Apr},
 note = {Aims
In comparison with a low linear energy transfer (LET) radiation, a high-LET radiation induces more complex DNA damage. This study wonders whether radiation-induced bystander effect (RIBE) is dependent of LET.

Materials and methods
Chinese hamster ovary CHO-9 cells and its subline EM-C11 cells (SSB repair deficient) and XR-C1 cells (DSB repair deficient) were irradiated by γ-rays, α-particles, or carbon ions with different LETs of 13, 30 and 70 keV/μm. Cell proliferation, cell death, DNA damage, cell cycle distribution and some protein expressions were measured with the cell counting kit-8 (CCK-8), colony formation, micronuclei (MN), flow cytometry and western blot, respectively.

Key findings
A series of cell responses were induced by these radiations in a LET-dependent manner, including proliferation inhibition, cell death, MN induction, G2/M phase arrest and the expression of γH2AX protein. These cell injuries were also depended on DNA repair capacity, and XR-C1 cells were the most sensitive to each radiation. Furthermore, when the cells were treated with the conditioned medium (CM) collected from irradiated CHO-9 cells, the MN induction and cell death response in the bystander cells of EM-C11 or XR-C1 increased along with LET of irradiation, and the bystander damage was easier to be induced in EM-C11 and XR-C1 cells than that in CHO-9 cells.

Significance
Both cellular DNA repair capacity and the LET value of radiation could deeply influence damage extents of not only the irradiated cells but also the bystander cells.},
 pages = {228--234},
 title = {Both irradiated and bystander effects link with DNA repair capacity and the linear energy transfer},
 volume = {222},
 year = {2019}
}