{"created":"2023-05-15T14:53:39.645651+00:00","id":73076,"links":{},"metadata":{"_buckets":{"deposit":"dc25ae4c-254d-42a2-9874-7ea9c852bc7c"},"_deposit":{"created_by":1,"id":"73076","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"73076"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00073076","sets":["10:28"]},"author_link":["720103","720098","720105","720104","720106","720100","720102","720101","720097","720099"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2018-12-03","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Many redox proteins possess cofactors, such as heme, FAD, and Fe-S cluster and the cofactors are involved in the chemical reactions of redox proteins. The redox reactions in proteins usually associate with the movement of hydrogen atoms and/or valence electrons through the cofactors. High-resolution neutron and X-ray structure analyses can provide the information about hydrogen atoms and valence electrons and are important for understanding molecular mechanisms of redox reactions in proteins. NADH-cytochrome b5 reductase (b5R) catalyzes the electron transfer from two-electron carriers of NADH to one-electron acceptor of cytochrome b5 (b5). High-resolution X-ray structure analyses have been reported for b5R and b5 from porcine liver. We prepared large crystals (> 1mm3) of the oxidized form of b5R for neutron diffraction experiments in the different pH conditions (6.5 and 7.5). We have successfully collected neutron diffraction data sets at high-resolutions of 1.40 Å (at iBIX in J-PARC) and 1.45 Å (at BIODIFF in FRM II). The diffraction data sets of iBIX were collected by the TOF-Laue method which utilizes white-pulsed neutrons. The integrated intensities of the iBIX data were corrected by applying wavelength normalization. The neutron structures obtained in different pH conditions show small structural changes in the hydrogen-bond network from FAD to the protein surface.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"Asian Crystallographic Association Conference 2018","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"平野, 優"}],"nameIdentifiers":[{"nameIdentifier":"720097","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"栗原, 和男"}],"nameIdentifiers":[{"nameIdentifier":"720098","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ostermann, Andreas"}],"nameIdentifiers":[{"nameIdentifier":"720099","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kusaka, Katsuhiro"}],"nameIdentifiers":[{"nameIdentifier":"720100","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kimura, Shigenobu"}],"nameIdentifiers":[{"nameIdentifier":"720101","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miki, Kunio"}],"nameIdentifiers":[{"nameIdentifier":"720102","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"玉田, 太郎"}],"nameIdentifiers":[{"nameIdentifier":"720103","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平野 優","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"720104","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"栗原 和男","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"720105","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"玉田 太郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"720106","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Neutron crystal structure studies of the oxidized form of NADH-cytochrome b5 reductase","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Neutron crystal structure studies of the oxidized form of NADH-cytochrome b5 reductase"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-12-10"},"publish_date":"2018-12-10","publish_status":"0","recid":"73076","relation_version_is_last":true,"title":["Neutron crystal structure studies of the oxidized form of NADH-cytochrome b5 reductase"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:33:07.258353+00:00"}