{"created":"2023-05-15T14:53:38.773892+00:00","id":73056,"links":{},"metadata":{"_buckets":{"deposit":"d17e8104-aec5-48c6-930b-4a3e09edaf39"},"_deposit":{"created_by":1,"id":"73056","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"73056"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00073056","sets":["10:28"]},"author_link":["719959","719963","719965","719961","719962","719958","719964","719960"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2018-11-24","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"塩橋とは酸性アミノ酸の負電荷と塩基性アミノ酸の正電荷の間に生じるイオン性相互作用であり、タンパク質の構造の安定化に寄与していると考えられている。さらに、糖質加水分解酵素の塩橋を消失させる事で酵素の低温活性が向上したという報告があるが、その一方で熱安定性が著しく低下するという問題点がある。本研究では塩橋を消失させるのではなく塩橋を弱めるという新たなアプローチにより、低温活性の向上並びに熱安定性の低下が抑制された糖質加水分解酵素の創出を目指した。\n本研究で用いた酵素は当研究グループが保有するシマミミズ由来マンナナーゼである。結晶構造をもとに4種の変異酵素R63K、R125K、R213K、R302Kを作製したところR302KがWTよりも低温側での活性が上昇し、R302-D300が形成する塩橋に注目した。そして、塩橋を消失させた変異酵素R302A、D300Nと熱安定性を比較したところ、熱安定性の低下が抑制されていることが明らかとなった。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第3回次世代生物研究会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"中, 裕規"}],"nameIdentifiers":[{"nameIdentifier":"719958","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平野, 優"}],"nameIdentifiers":[{"nameIdentifier":"719959","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中澤, 昌美"}],"nameIdentifiers":[{"nameIdentifier":"719960","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"阪本, 龍司"}],"nameIdentifiers":[{"nameIdentifier":"719961","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"玉田, 太郎"}],"nameIdentifiers":[{"nameIdentifier":"719962","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"上田, 光宏"}],"nameIdentifiers":[{"nameIdentifier":"719963","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平野 優","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"719964","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"玉田 太郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"719965","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Eisenia fetida由来マンナナーゼ活性に及ぼす塩橋の影響について","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Eisenia fetida由来マンナナーゼ活性に及ぼす塩橋の影響について"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-11-27"},"publish_date":"2018-11-27","publish_status":"0","recid":"73056","relation_version_is_last":true,"title":["Eisenia fetida由来マンナナーゼ活性に及ぼす塩橋の影響について"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:33:19.361450+00:00"}