{"created":"2023-05-15T14:53:36.138598+00:00","id":72996,"links":{},"metadata":{"_buckets":{"deposit":"7652e4a8-1b3e-4dc7-b771-714be4d39859"},"_deposit":{"created_by":1,"id":"72996","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"72996"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00072996","sets":["10:28"]},"author_link":["719328","719327","719332","719338","719326","719334","719329","719330","719336","719331","719335","719333","719337"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2018-10-13","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose: Tissue Factor (TF) is a transmembrane protein and its overexpression is associated with increased tumor growth, tumor angiogenesis and metastatic potential in many malignancies, including pancreatic cancer. Near infrared photoimmunotherapy (NIR-PIT) is a new target-cell-specific cancer treatment that enables highly selective cell death after systemic administration of a photosensitizer-antibody conjugate followed by subsequent NIR light exposure. In this study, we describe the photoimmunotherapeutic effect induced by a rat IgG2b anti-TF monoclonal antibody (anti-TF mAb), conjugated to the photosensitizer, Indocyanine green (ICG), in a TF-expressing BxPC-3 pancreatic cancer model. Materials and methods: Specific binding of the anti-TF-ICG antibody to the TF-expressing BxPC-3 pancreatic cancer cell line was examined by fluorescence microscopy. NIR-PIT-induced cell death was determined by using cell viability imaging assay after exposure of the cells to NIR light. Anti-TF-ICG antibody accumulation in xenograft tumor was acquired in in vivo longitudinal fluorescence imaging. To conduct NIR-PIT, tumor-bearing mice were separated into 5 groups: (1) 100 μg of anti- TF-ICG i.v. administration followed by NIR light exposure (50 J/cm2) for two consecutive days (Day 1 and 2); (2) NIR light exposure (50 J/cm2) only for two consecutive days (Day 1 and 2); (3) 100 μg of anti-TF-ICG i.v. administration; (4) 100 μg of unlabeled anti-TF i.v. administration; (5) the untreated control. Biweekly tumor volume measurements, histopathological and immunohistochemical (IHC) analyses of tumors at 3 days post-administration of anti-TF-ICG antibody were performed to monitor the effect of treatments. Results: Dead cells were obviously seen with cell viability imaging assay. Tumor growth was significantly inhibited by NIR-PIT compared with the other control groups (P < 0.01) for 27 days. Tumors received NIR-PIT showed the evidence of necrotic cell death associated features on H&E staining and decreased cell proliferation marker Ki-67-positive cells on IHC examination. Conclusion: The anti-TF-ICG antibody is suitable as a photosensitizer-antibody conjugate for NIR-PIT. Furthermore, NIR-PIT with the anti-TF-ICG antibody conjugate promises to open a new avenue for treatment of TF-expressing pancreatic cancer and that could be ultimately applicable in clinical use.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":" Annual Congress of the European Association of Nuclear Medicine 2018","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ｕ, Ｗｉｎｎ Ａｕｎｇ"}],"nameIdentifiers":[{"nameIdentifier":"719326","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsuji, Atsushi"}],"nameIdentifiers":[{"nameIdentifier":"719327","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sugyo, Aya"}],"nameIdentifiers":[{"nameIdentifier":"719328","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sudo, Hitomi"}],"nameIdentifiers":[{"nameIdentifier":"719329","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takashima, Hiroki"}],"nameIdentifiers":[{"nameIdentifier":"719330","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yasunaga, Masahiro"}],"nameIdentifiers":[{"nameIdentifier":"719331","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsumura, Yasuhiro"}],"nameIdentifiers":[{"nameIdentifier":"719332","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Higashi, Tatsuya"}],"nameIdentifiers":[{"nameIdentifier":"719333","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｕ Ｗｉｎｎ Ａｕｎｇ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"719334","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻 厚至","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"719335","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"須尭 綾","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"719336","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"須藤 仁美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"719337","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"東 達也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"719338","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Near-infrared Photoimmunotherapy Targeting Pancreatic Cancer With Tissue Factor Antibody","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Near-infrared Photoimmunotherapy Targeting Pancreatic Cancer With Tissue Factor Antibody"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-11-08"},"publish_date":"2018-11-08","publish_status":"0","recid":"72996","relation_version_is_last":true,"title":["Near-infrared Photoimmunotherapy Targeting Pancreatic Cancer With Tissue Factor Antibody"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:33:53.100202+00:00"}