@misc{oai:repo.qst.go.jp:00072981,
 author = {勝部, 孝則 and 王, 冰 and 田中, 薫 and 二宮, 康晴 and 平川, 博一 and 劉, 翠華 and 丸山, 耕一 and Ｇｕｉｌｌａｕｍｅ, Ｖａｒｅｓ and Liu, Qiang and 中島, 徹夫 and 藤森, 亮 and 根井, 充 and 勝部 孝則 and 王 冰 and 田中 薫 and 二宮 康晴 and 平川 博一 and 劉 翠華 and 丸山 耕一 and Ｇｕｉｌｌａｕｍｅ Ｖａｒｅｓ and 中島 徹夫 and 藤森 亮 and 根井 充},
 month = {Sep},
 note = {Purpose: Astronauts develop psychological stress　(PS) during space flight because of enclosed environment, zero gravity and altered light-dark cycles. At the same time, they are exposed to cosmic rays including high atomic number and energy (HZE) particles such as iron-56 (Fe) ions. Exposure to either PS or ionizing radiation (IR) could cause detrimental effects on humans. A pioneering study showed that chronic restraint-induced PS (CRIPS) could attenuate Trp53 functions and increase carcinogenesis induced by low LET gamma-rays in Trp53-heterozygous (Trp53+/-) mice. To elucidate possible modification effects from CRIPS on high linear energy transfer (LET) HZE particles-induced health consequences, Trp53+/- mice were given both CRIPS and accelerated Fe ions. Materials and Methods: Six-week-old male Trp53+/- C57BL/6N mice were restrained 6 hours per day for consecutive 28 days, and total-body irradiation (TBI) at a dose of 0.1 or 2 Gy was given on the 8th day. Metaphase chromosome spreads prepared from splenocytes at the end of the 28-day restraint regimen were painted with the fluorescence in situ hybridization (FISH) probes for chromosomes 1 (green), 2 (red) and 3 (yellow). Results and Conclusions: Induction of PS in our experimental model was confirmed by increase in urinary corticosterone level on the 7th day of restraint regimen. CRIPS reduced splenocyte number per spleen at the end of the 28-day restraint regimen but TBI of Fe ions didn’t. Observation of chromosomal aberrations in splenocytes is ongoing. At the annual meeting, results on the possible modification effects from CRIPS on genotoxicity of Fe ions in splenocytes will be introduced and discussed. Acknowledgement: This work was partially supported by both the Ministry of Education, Culture, Sports, Science and Technology Grant-in-Aid for Scientific Research on Innovative Areas, Grant Numbers JP15K21745 and 5H05935 “Living in Space” and 3 HIMAC Research Project Grants (22B258, 14J286 and 16J295). The expert technical assistance and administrative support of Ms. Hiromi Arai, Mr. Sadao Hirobe, Ms. Mikiko Nakajima, and Ms. Yasuko Morimoto are gratefully acknowledged. For correspondence: katsube.takanori@qst.go.jp; nenoi.mitsuru@qst.go.jp
Key words: chronic restrain-induced psychological stress; total body Fe irradiation; fluorescence in situ hybridization (FISH); mouse restraint model., The 64th Annual Radiation Research Society Meeting},
 title = {Effects from chronic restraint-induced stress and total body Fe-particle irradiation on the induction of chromosomal aberrations in splenocytes of Trp53-heterozygous mice},
 year = {2018}
}