@misc{oai:repo.qst.go.jp:00072928, author = {Miyagi, Atsuko and Saimaru, Takuya and Harigai, Nozomi and 大野, 豊 and 長谷, 純宏 and Toshiki, Ishikawa and Yamaguchi, Masatoshi and 大野 豊 and 長谷 純宏}, month = {Sep}, note = {Oxalate, which is contained in many plant species, easily combines cationic ions such as calcium, iron, and magnesium ions. It is important agricultural topic to reduce oxalate content in crops, because excess uptake of oxalate causes mineral deficiency or urinary syndrome for human and livestock. Rice straw can be used as a livestock feed. However, rice accumulates oxalate in leaves and stems which consist mostly of rice straw. For the reduction of oxalate content in rice leaves, we performed screening of low oxalate rice plants from seeds irradiated by carbon ion beam. The CE-MS (Capillary Electrophoresis-Mass Spectrometry) measurement of oxalate contents in leaves obtained from ion beam-irradiated seeds (M2) showed that low-oxalate plants which contained less than one tenth of oxalate content in comparison with those in non-irradiated plants was obtained. Multivariate analyses in the basis of oxalate and other metabolite data sets revealed that amino acids (except for alanine, glutamate, and aspartate) were accumulated in low oxalate rice plants, although organic acids and phosphate compounds were decreased. These suggested that carbon flow via isocitrate affected oxalate accumulation. In the M3 mutants, several lines showed decreased oxalate contents (60% of non-irradiated plant) and could grow normally., 16th International Symposium on Rice Functional Genomics}, title = {Screening of low oxalate rice plants from ion beam-irradiated seeds}, year = {2018} }