@misc{oai:repo.qst.go.jp:00072927,
 author = {大野, 豊 and Hiroyuki, Ichida and Ryouhei, Morita and 野澤, 樹 and Tomoko, Abe and Hiroshi, Kato and 長谷, 純宏 and 大野 豊 and 野澤 樹 and 長谷 純宏},
 month = {Sep},
 note = {Ion beams are recognized as alternative mutagens for plant and microbe breeding because they are thought to cause mutations by distinct mechanism from chemical mutagens or gamma rays. Valuable mutants have been successfully generated by ion beam irradiation not only in ornamental plants but also in crops such as rice. The characteristics of the ion-beam-induced mutations had been analyzed using specific marker genes; however, little is known about how many and what type of mutations are induced by ion beams in genomic level. Here, we conducted exome analysis of DNA samples of mutants identified in a carbon ion beam-irradiated rice population to understand the properties of mutations caused by ion beams at a genomic level.
Rice seeds (Nipponbare) were irradiated with carbon ion beams (320 MeV 12C6+ at 40 Gy) accelerated by the cyclotron in QST Takasaki. Five mutant lines (2 dwarfs and 3 early heading date mutants) were selected and subjected to the exome analysis. As a result, we identified a total of 56 mutations including 24 single nucleotide variations and 28 insertions/deletions in the 5 lines. A large deletion (33.6k bp) and an inversion (534K bp) were also detected. In addition, we identified 6 mutations that potentially have high impact on protein function (frameshift or exon loss variants). Strong candidate genes that possibly link to the mutant phenotype were found in 4 lines out of 5 lines, suggesting that the combination of ion beam irradiation and genome analysis is helpful to narrow down a candidate gene responsible for a mutant phenotype., 16th International Symposium on Rice Functional Genomics},
 title = {Effect of ion beams on rice genome sequence revealed by exome analysis},
 year = {2018}
}