{"created":"2023-05-15T14:53:27.420378+00:00","id":72797,"links":{},"metadata":{"_buckets":{"deposit":"52b50918-4d80-4d09-94ca-74b9ad6a161c"},"_deposit":{"created_by":1,"id":"72797","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"72797"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00072797","sets":["10:28"]},"author_link":["717220","717227","717221","717223","717230","717224","717225","717228","717229","717222","717226"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2018-05-17","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"【目的】HER2陽性乳癌はHER2陰性乳癌に比べ再発や転移を起こす例が多く、予後が悪い。一方、microRNA21(miRNA21)は癌抑制遺伝子を負に制御するmiRNAとして多くのヒト腫瘍で過剰発現している。本研究では、炭素線照射と分子標的薬Lapatinibとの併用によるHER2陽性乳癌細胞に対する細胞死誘導、miRNA21との関わりなどの分子メカニズムについて調べる。\n【方法】HER2陽性乳癌細胞BT474、SKBR3に対して、炭素線照射単独或はLapatinib との併用処置を行い、FACSAria, FACS Callibur, Caspase-Gloや Real-Time PCRを用いて、細胞生存率、apoptosis誘導、細胞死（apoptosisやautophagy）関連遺伝子発現変化、miRNA21発現変化、癌幹細胞のspheroid形成能変化、細胞周期の変化などについてX線照射のものと比較検討した。\n【結果】炭素線、X線照射単独に比べ、炭素線照射とLapatinib との併用処置はHER2陽性乳癌細胞のapoptosisを顕著に誘導し、autophagy関連遺伝子Beclin1, LC3の発現を有意に上昇させ、細胞生存率を有意に低下させた。また、miRNA解析ではmiRNA21発現はX線照射では有意に増加するのに対し、炭素線照射とLapatinib との併用は顕著に抑制された。細胞周期解析ではG2/M期延長、癌幹細胞解析では炭素線とLapatinibとの併用により癌幹細胞ESA+/CD24-の割合が顕著に減少し、spheroid形成能を有意に低下させることが認められた。\n【結論】以上より、炭素線照射とLapatinibとの併用はmiRNA21発現や癌幹細胞を顕著に抑制し、apoptosis,　autophagyを誘導することにより有効にER2陽性乳癌細胞を殺傷することが示唆された。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第26回日本乳癌学会学術総会 ","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"崔, 星"}],"nameIdentifiers":[{"nameIdentifier":"717220","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"林光弘"}],"nameIdentifiers":[{"nameIdentifier":"717221","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｋｅｕｍ, Ｗｏｏｎｇ Ｓｅｏｐ"}],"nameIdentifiers":[{"nameIdentifier":"717222","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"新田, 祐樹"}],"nameIdentifiers":[{"nameIdentifier":"717223","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鈴木, 雅雄"}],"nameIdentifiers":[{"nameIdentifier":"717224","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"堀本, 義哉"}],"nameIdentifiers":[{"nameIdentifier":"717225","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"崔 星","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"717226","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｋｅｕｍ Ｗｏｏｎｇ Ｓｅｏｐ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"717227","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"新田 祐樹","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"717228","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鈴木 雅雄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"717229","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"堀本 義哉","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"717230","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"HER2陽性乳癌細胞に対するLapatinib併用とmiRNA21制御による炭素線増感メカニズム","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"HER2陽性乳癌細胞に対するLapatinib併用とmiRNA21制御による炭素線増感メカニズム"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-05-21"},"publish_date":"2018-05-21","publish_status":"0","recid":"72797","relation_version_is_last":true,"title":["HER2陽性乳癌細胞に対するLapatinib併用とmiRNA21制御による炭素線増感メカニズム"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:35:57.568764+00:00"}