{"created":"2023-05-15T14:53:25.940373+00:00","id":72763,"links":{},"metadata":{"_buckets":{"deposit":"a57546ea-1345-4746-a99a-564ed3846fb4"},"_deposit":{"created_by":1,"id":"72763","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"72763"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00072763","sets":["10:28"]},"author_link":["716736","716738","716741","716737","716734","716742","716732","716733","716740","716739","716735","716731"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2018-04-21","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background/Aims: [11C]Choline has been extensively used clinically for imaging prostate cancer [1]. [11C]Choline is prepared by 11C-methylation of [11C]methyl iodide and 2-dimethylaminoethanol (DMAE). It is important to determine accurately the quantity of DMAE in the final product. The most common method for the determination of the substance of [11C]choline or DMAE was by co-injection of carrier-choline or DMAE method, and by two separate analysis LC system and gas chromatography [2-4]. The quality control procedures for 11C-labeled radiopharmaceuticals is acquired rapid assessment due to short half-life radionuclide (about 20 min). Thus, we developed a rapid and simultaneous measurement method for the molar radioactivity, radiochemical purity, and chemical impurity of the [11C]choline injection using the radio-HPLC coupled with the corona-charged aerosol detector (CAD). Methods: The molar radioactivity, radiochemical purity, and chemical impurity (DMAE) of the [11C]choline injection was measured using the radio-HPLC-CAD with the post-column method. We also validated the measurement of choline and DMAE using this HPLC, and evaluated these parameters of the accuracy, precision, specificity, quantitation of limit, and linearity. Results: The molar radioactivity, radiochemical purity, and chemical impurity were over 130 GBq/μmol (over 0.1 μg/mL) at end of synthesis (EOS), over 95% at EOS, and less than 0.5 μg/mL of DMAE, respectively. In the validation, the percentages of recovery of choline and DMAE were within 100±5%. The RSD of choline and DMAE were less than 10%. Resolution between obtained choline and DMAE peak was over 1.5. The limit of quantitation of choline and DMAE was 0.1 and 0.5 μg/mL, respectively. The coefficient of correlation (R2) of choline (0.1-50 μg/mL) and DMAE (0.5-50 μg/mL) found to be >0.9999. Conclusions: We developed and optimized the simultaneous measurement method for the molar radioactivity, radiochemical purity, and chemical impurity of the [11C]choline injection using the radio-HPLC-CAD with the post-column method.\nReferences: [1] Giovacchini G, et al. Eur J Nucl Med Mol Imaging. 2017;44:1751-76. [2] Mishani E, et al. Nucl Med Biol. 2002;39:359-62. [3] Shao X, et al. Appl Radiat Isot. 2011;69:403-9. [4] Biasiotto G, et al. Med Chem. 2012;8:1182-9.\n\\n\n(Abstracts are to be no more than 300 words. References, Title and authors details are not included in the word count. Abstracts should not contain bullet points.)","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"12th World Congress of the World Federation of Nuclear Medicine and Biology","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hashimoto, Hiroki"}],"nameIdentifiers":[{"nameIdentifier":"716731","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawamura, Kazunori"}],"nameIdentifiers":[{"nameIdentifier":"716732","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furutsuka, Kenji"}],"nameIdentifiers":[{"nameIdentifier":"716733","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kariya, Kaori"}],"nameIdentifiers":[{"nameIdentifier":"716734","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nengaki, Nobuki"}],"nameIdentifiers":[{"nameIdentifier":"716735","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhang, Ming-Rong"}],"nameIdentifiers":[{"nameIdentifier":"716736","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"橋本 裕輝","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"716737","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"河村 和紀","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"716738","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古塚 賢士","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"716739","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"狩谷 香","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"716740","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"念垣 信樹","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"716741","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"張 明栄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"716742","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"The simultaneous measurement method for the molar radioactivity, radiochemical purity, and chemical impurity of the [11C]choline injection","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The simultaneous measurement method for the molar radioactivity, radiochemical purity, and chemical impurity of the [11C]choline injection"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-04-27"},"publish_date":"2018-04-27","publish_status":"0","recid":"72763","relation_version_is_last":true,"title":["The simultaneous measurement method for the molar radioactivity, radiochemical purity, and chemical impurity of the [11C]choline injection"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:36:20.403982+00:00"}