{"created":"2023-05-15T14:53:17.956882+00:00","id":72587,"links":{},"metadata":{"_buckets":{"deposit":"89b8945b-4a8f-4fa2-b8a8-064e083a142d"},"_deposit":{"created_by":1,"id":"72587","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"72587"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00072587","sets":["10:28"]},"author_link":["714923","714928","714926","714924","714922","714925","714927"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2017-12-13","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":" 本研究では、放線菌のミコール酸含有細菌に対する二次代謝産物生産応答に関与する遺伝子を、突然変異株スクリーニングによる逆遺伝学的手法を用いて同定することを目的として行った。Streptomyces coelicolor A3(2)株を対象として、炭素イオンビームを照射し、突然変異株ライブラリーを作製した。突然変異株ライブイラリーから、赤色色素の非誘導生産を指標に候補株の選抜を行った。その結果、これまでに約150,000株から対峙培養による確認試験、最小培地での生育能、気中菌糸形成能、高塩濃度条件下における赤色色素生産能などを指標に順次、選抜した。その結果、野生株とほぼ同等の形態を有した株を７株得ることに成功した。現在、７株を含めた合計16株の変異株について、定法に基づき次世代シーケンサ―を用いたゲノムシーケンスを行い、変異点の同定を行う予定である。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"QST高崎サイエンスフェスタ2017","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"栁澤, 昌臣"}],"nameIdentifiers":[{"nameIdentifier":"714922","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"浅水, 俊平"}],"nameIdentifiers":[{"nameIdentifier":"714923","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐藤, 勝也"}],"nameIdentifiers":[{"nameIdentifier":"714924","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大野, 豊"}],"nameIdentifiers":[{"nameIdentifier":"714925","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"尾仲, 宏康"}],"nameIdentifiers":[{"nameIdentifier":"714926","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐藤 勝也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"714927","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大野 豊","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"714928","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"重イオンビーム照射変異株を用いた異属細菌刺激による放線菌二次代謝活性化機構の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"重イオンビーム照射変異株を用いた異属細菌刺激による放線菌二次代謝活性化機構の解析"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-12-19"},"publish_date":"2017-12-19","publish_status":"0","recid":"72587","relation_version_is_last":true,"title":["重イオンビーム照射変異株を用いた異属細菌刺激による放線菌二次代謝活性化機構の解析"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:38:09.472988+00:00"}