@misc{oai:repo.qst.go.jp:00072291,
 author = {Wozny, A-S. and Gersende, ALPHONSE and Vares, G. and 藤森, 亮 and 中島, 徹夫 and Rodriguez-Lafrasse, C. and 藤森 亮 and 中島 徹夫},
 month = {Apr},
 note = {Head and neck squamous cell carcinoma (HNSCC) are resistant to standard treatments, partly due to the presence of Cancer Stem Cells (CSCs) localized in  hypoxic niches. The protein HIF-1α (Hypoxia-Inducible Factor 1α）is considered as the major transcriptional regulator of the cellular response to oxygen homeostasis. We previously showed that inhibition of HIF-1α lead to the decrease of HNSCC-CSC survival after carbon ion (GANIL, France (75MeV/n)) and photon irradiations in hypoxic conditions. This proposal aimed of clarifying the role of HIF-1α in the response of HNSCC-CSCs. After inhibition of HIF-１α with a siRNA, DNA repair and epithelio-mesenchymal transition(EMT) were studied in response to carbon ion(290MeV/n-SOBP) and photon (250kV) irradiations in normoxic or hypoxic(1% O2) conditions. Radiosentization observed after HIF-1αinhibition is associated with a significant increase of residual DSBs in response to both types of radiation. Furthermore, under hypoxia, HIF-1αinhibition is associated with a low proportion of migrating and invasive cells, confirming the role of HIF-1α in EMT. In summary,HIF-1α participates to radioresistance by increasing cell survival, DNA repair and invasiveness and contributes to tumor escape. These preliminary results will be confirmed during the next beam time., H28年度HIMAC共同利用研究成果発表会},
 title = {Role of HIF-1α in the response of HNSCC cancer stem cells after carbon ion exposures},
 year = {2017}
}