@misc{oai:repo.qst.go.jp:00072157,
 author = {中島, 徹夫 and Vares, Guillaume and 二宮, 康晴 and 王, 冰 and 勝部, 孝則 and 田中, 薫 and 劉, 翠華 and 平川, 博一 and 丸山, 耕一 and 藤森, 亮 and 根井, 充 and 中島 徹夫 and 二宮 康晴 and 王 冰 and 勝部 孝則 and 田中 薫 and 劉 翠華 and 平川 博一 and 丸山 耕一 and 藤森 亮 and 根井 充},
 month = {Feb},
 note = {Radiation induces many molecular alterations in organisms. Biological markers, which indicate radiation effects and are used for predicting late effects in the future, are expected to be identified. This study purpose is exploration of useful biological markers to evaluate radiation effects. Many approaches at the molecular level such as transcriptomics and proteomics have been performed. Recently metabolomics, advanced omics analysis of metabolite, has attracted much attention. Low molecular weight metabolites, which are more directly related to biological activities in organisms than DNA or proteins, are evaluated in metabolomics. Here, metabolic analyses were performed in livers of mice irradiated at 0.1, 0.5 and 2 Gy with X-rays. Dose-dependent or dose-specific alterations were evaluated. In addition to it, investigating differences of biomarkers due to radiation quality, specific alteration in metabolites was compared with that in the case of Fe-ion irradiation.
Mice (C57BL/6J) were irradiated with X-rays. Metabolites in the liver were analyzed by capillary electrophoresis-time-of-flight mass spectrometry (CE-TOFMS) one month after irradiation. In the analyses, a total of 281 metabolites (109 anions and 172 cations) were identified in the livers of mice irradiated at doses of 0.1, 0.5 and 2 Gy with X-rays. Principal component analysis (PCA) was performed to reveal differences in the metabolite profiles of the four groups, which consisted of the control, 0.1, 0.5 and 2 Gy irradiation. In PCA, distribution of four groups had no clear differences in the plot; however, the group of irradiation at 0.1Gy was separated from that at 2Gy along PC2. In addition, significantly altered metabolites to the control were selected from the irradiation groups and specific trends were evaluated. One month after irradiation, 4, 8 and 16 metabolites were significantly altered in irradiated groups at doses of 0.1Gy, 0.5Gy and 2Gy, respectively, compared to the levels in the control. Significantly altered metabolites included free amino acids. Particularly, changes in branched chain amino acids (BCAA) such as Ile or Leu could influence the de novo synthesis of glutamate or GABA in brains via blood. Changes in free amino acids after Fe-ion irradiation (0, 0.1 and 2 Gy) were also analyzed. These results will be discussed in the viewpoint of amino acid metabolism and usage as biomarkers for evaluating radiation effects., 第7回国際放射線神経生物学会大会　ISRN-2017-Yuzawa},
 title = {Changes in free amino acids in mice exposed to X-ray or Fe-ion radiation.},
 year = {2017}
}