{"created":"2023-05-15T14:52:49.043097+00:00","id":72112,"links":{},"metadata":{"_buckets":{"deposit":"414db407-c3d0-463e-ac51-894b50e6e819"},"_deposit":{"created_by":1,"id":"72112","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"72112"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00072112","sets":["10:28"]},"author_link":["710169","710167","710166","710165","710168","710164"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2016-08-08","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The fully human monoclonal antibody KMTR2 acts as a strong direct agonist for TNF-related apoptosis-inducing ligand receptor 2 (TRAIL-R2). To investigate the mechanism of direct agonistic activity induced by KMTR2, the crystal structure of the extracellular region of TRAIL-R2 and a Fab fragment derived from KMTR2 (KMTR2-Fab) was determined to 2.1 Å resolution. Two KMTR2-Fabs assembled with the CDR2 region of the light chain via two-fold crystallographic symmetry, suggesting that the KMTR2-Fab assembly tended to enhance TRAIL-R2 oligomerization. A single mutation at Asn53 to Arg located at the two-fold interface in the KMTR2 resulted in a loss of its apoptotic activity. These results indicate that the strong agonistic activity, such as apoptotic signaling and tumor regression, induced by KMTR2 is attributed to TRAIL-R2 superoligomerization induced by the interdimerization of KMTR2.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第5回回折構造生物国際シンポジウム(ISDSB2016)","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"玉田, 太郎"}],"nameIdentifiers":[{"nameIdentifier":"710164","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"新見, 大輔(協和発酵キリン株式会社 創薬技術研究所)"}],"nameIdentifiers":[{"nameIdentifier":"710165","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"池田, 昌弘(協和発酵キリン株式会社 免疫アレルギー研究所)"}],"nameIdentifiers":[{"nameIdentifier":"710166","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"森, 英治(協和発酵キリン株式会社 研究開発企画部)"}],"nameIdentifiers":[{"nameIdentifier":"710167","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"元木, 一宏(協和発酵キリン株式会社 がん研究所)"}],"nameIdentifiers":[{"nameIdentifier":"710168","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"玉田 太郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"710169","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"TRAIL-R2 Superoligomerization Induced by Human Monoclonal Agonistic Antibody KMTR2","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"TRAIL-R2 Superoligomerization Induced by Human Monoclonal Agonistic Antibody KMTR2"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-01-05"},"publish_date":"2017-01-05","publish_status":"0","recid":"72112","relation_version_is_last":true,"title":["TRAIL-R2 Superoligomerization Induced by Human Monoclonal Agonistic Antibody KMTR2"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:43:40.872494+00:00"}