{"created":"2023-05-15T14:52:46.182228+00:00","id":72047,"links":{},"metadata":{"_buckets":{"deposit":"cfda3cbd-07be-4c7a-ae2b-221b8265923c"},"_deposit":{"created_by":1,"id":"72047","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"72047"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00072047","sets":["10:28"]},"author_link":["709578","709580","709585","709582","709581","709584","709586","709576","709575","709574","709583","709577","709579"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2016-10-27","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Direct hits in the nucleus by radiation are known as the primary cause of th e various radio-biological effects. However, the cells will have equal chanc es of being exposed in the cytoplasm, and results in damages that may activa te various signaling pathways that mediates defensive response. We examined the velocity of DNA double-strand break (DSB) repair in microbeam irradiated  human normal fibroblast, WI-38 cells that were targeted in the nucleus, cyt oplasm, or both nucleus/cytoplasm using SPICE-NIRS microbeam. Cells were fix ed at various time points of 1hr to 24hr post hours irradiation, then immuno -stained against g-H2AX to quantify the residual DSB/nucleus from the obtain ed microscopic images. Microbeam irradiation significantly induced g-H2AX, w hich were proportional to the number of protons per nucleus. With the cells irradiated with 250 protons per nucleus, we found less residual g-H2AX in th e cells with additional 200 protons in the cytoplasm at 8hr and 16hr post ir radiation. In contrary, cytoplasm irradiation alone showed slightly more g-H 2AX than the control. Taken together, radiation exposure in cytoplasm enhanc es DSB repair that is mediated by the cytoplasmic damage.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"日本放射線影響学会第59回大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小西, 輝昭"}],"nameIdentifiers":[{"nameIdentifier":"709574","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小林, 亜利紗"}],"nameIdentifiers":[{"nameIdentifier":"709575","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古澤, 佳也"}],"nameIdentifiers":[{"nameIdentifier":"709576","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"劉, 翠華"}],"nameIdentifiers":[{"nameIdentifier":"709577","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"及川, 将一"}],"nameIdentifiers":[{"nameIdentifier":"709578","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ahbrizal, Farizal Tengku Ahmad Tengku"}],"nameIdentifiers":[{"nameIdentifier":"709579","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Autsavapromporn, Narongchai"}],"nameIdentifiers":[{"nameIdentifier":"709580","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wang, Jun"}],"nameIdentifiers":[{"nameIdentifier":"709581","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小西 輝昭","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"709582","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小林 亜利紗","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"709583","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古澤 佳也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"709584","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"劉 翠華","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"709585","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"及川 将一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"709586","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Microbeam induced cytoplasmic damage triggers activation of DNA double-stran d break repair","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Microbeam induced cytoplasmic damage triggers activation of DNA double-stran d break repair"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-10-31"},"publish_date":"2016-10-31","publish_status":"0","recid":"72047","relation_version_is_last":true,"title":["Microbeam induced cytoplasmic damage triggers activation of DNA double-stran d break repair"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:44:26.185432+00:00"}