@misc{oai:repo.qst.go.jp:00071734,
 author = {Hasegawa, Sumitaka and Li, Huizi and Morokoshi, Yukie and Furukawa, Takako and Saga, Tsuneo and 長谷川 純崇 and 李 惠子 and 諸越 幸恵 and 古川 高子 and 佐賀 恒夫},
 month = {Jun},
 note = {111In-labeled trastuzumab modified with nuclear localizing signal (NLS) peptides (111In-trastuzumab-NLS) is a promising therapeutic agent for cancer cells overexpressing HER2 because it can efficiently deliver an Auger electron emitter 111In into tumor cell nucleus and 111In is highly toxic when it decays in the nucleus. However, to further improve its therapeutic efficacy, better understanding of cellular responses to 111In-trastuzumab-NLS is required. The aim of this study is to identify gene expression signatures of cells treated with 111In-trastuzumab-NLS using microarray technology.
\nTrastuzumab modified with approximately 10 NLS peptides per antibody was generated. Microarray analyses were performed using RNAs isolated from human breast cancer SKBR3 cells treated for 7 days with the treatments including 231.25 MBq of trastuzumab-NLS. Untreated SKBR3 cells were used as control. The identified genes were imported to the Ingenuity Pathway Analysis (IPA) to characterize gene expression and biofunctional changes induced by 111In-trastuzumab-NLS.
\nMicroarray data showed that more than 1,200 probes were up-regulated and down-regulated in treatment of 111In-trastuzumab-NLS in comparison with control and revealed that 338 and 520 probes were specifically up-regulated and down-regulated in treatment of 111In-trastuzumab-NLS, respectively. IPA detected molecular and cellular functions affected by treatment of 111In-trastuzumab-NLS, including cell-to-cell signaling and interaction and cell death/survival.
\nOur microarray study identified gene expression signatures of human breast cancer cells treated with 111In-trastuzumab-NLS. These data may be useful for enhancing therapeutic efficacy of 111In-trastuzumab-NLS., 2015 Society of Nuclear Medicine and Molecular Imaging Annual Meeting},
 title = {Microarray analysis of human breast cancer cells treated with 111In-trastuzumab modified with nuclear localizing signal peptides},
 year = {2015}
}