@misc{oai:repo.qst.go.jp:00071685,
 author = {藤森, 亮 and 高橋, 桃子 and 平川, 博一 and 矢島, 浩彦 and 中島, 菜花子 and 藤森 亮 and 高橋 桃子 and 平川 博一 and 矢島 浩彦 and 中島 菜花子},
 month = {May},
 note = {The aim of this paper is to obtain human glioblastoma cells A172 expressing stem cell-related protein and comparison of radiosensitivity in these cells with X-rays and carbon beam. Human monolayer-type A172 glioblastoma cells were maintained in normal medium with 10% bovine serum. In order to obtain sphere-type A172 cells the medium was replaced with serum-free medium supplemented with growth factors. Both types of A172 cells were irradiated with either X-rays or carbon ion beams and their radiosensitivity was evaluated. Serum-free medium induced expression of stem cell-related proteins in A172 cells along with the neurosphere-like appearance. These sphere-type cells were found resistant to both X-rays and carbon ion beams. Phosphorylation of histone H2A family member X persisted for a longer period in the cells exposed to carbon ion beams than in those exposed to X-rays and it disappeared quicker in the sphere type than in the monolayer type. Relative radioresistance of the sphere type cells was smaller for carbon ion beams than for X-rays. We demonstrated that glioblastoma A172 cells with induced stem cell-related proteins turned resistant to irradiation. Accelerated heavy ion particles may have advantage over X-rays in overcoming the tumor resistance due to cell stemness., ICRR2015},
 title = {Carbon ion beam is more effective to induce cell death in sphere-type A172 human glioblastoma cells compared with X-rays},
 year = {2015}
}