{"created":"2023-05-15T14:52:29.402359+00:00","id":71665,"links":{},"metadata":{"_buckets":{"deposit":"fea1eba1-5a68-4e35-9801-df332d86e881"},"_deposit":{"created_by":1,"id":"71665","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"71665"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00071665","sets":["10:28"]},"author_link":["705148","705145","705147","705150","705154","705152","705149","705153","705151","705146"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2015-05-28","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Since 1994, a Phase I/II clinical study and radiotherapy have been carrying out using carbon-ion beams generated with the Heavy Ion Medical Accelerator in Chiba (HIMAC) at National Institute of Radiological Sciences. Now we constructed the new treatment facility for the advanced carbon-ion therapy applying a 3D fast spot scanning system with pencil beams. To identify the biological effects of a spot scanning carbon-ion beams in vitro, we demonstrated cell-killing effect of human tumor cell lines using the spot scanning irradiation of carbon-ion microbeams generated with the Takasaki Ion Accelerators for Advanced Radiation Application (TIARA) in Japan Atomic Energy Agency. We irradiated either 4-cell lines with wild-type P53 or 4-cell lines with mutated-type P53 using the TIARA microbeams collimated by 20µm in diameter. We can easily estimate the number of the directly irradiated cells to be 0.01% level of the total cells on the dish using the highly controlled microbeam irradiation system. The percent survival in the cells with wild-type P53 was around 90%, while almost 100% was observed in the cells with mutated-type P53. We can expect the percent survival of 99.99% level when the cell-killing effect was induced in directly irradiated 0.01% cells, assuming no bystander effect. However, the percent in the cells with wild-type P53 was significantly low beyond our expectation. Moreover, the percent returned to 100% when using a specific inhibitor of gap-junction mediated cell-cell communication. The results are consistent with the data using the carbon-ion broad beams with the shielding method at HIMAC, which irradiation was carried out using 4 different protocols; (1) All cells were irradiated; (2) Irradiated and unirradiated cells were pooled in a 1:1 ratio as a single culture; (3) Half of cells were irradiated using beam stopper; and (4) Half of cells were irradiated with a specific inhibitor of gap-junction. Our overall results showed that bystander cell-killing effect was observed in the cells with wild-type P53, but not in the P53-mutated cells. There is clear evidence that the spot scanning irradiation system of carbon ions enables the enhanced cell killing in cells with wild-type P53 gene via gap-junction mediated bystander effect.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"15th International Congress of Radiation Research","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鈴木, 雅雄"}],"nameIdentifiers":[{"nameIdentifier":"705145","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"舟山, 知夫"}],"nameIdentifiers":[{"nameIdentifier":"705146","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"横田, 裕一郎"}],"nameIdentifiers":[{"nameIdentifier":"705147","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鈴木, 芳代"}],"nameIdentifiers":[{"nameIdentifier":"705148","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"池田, 裕子"}],"nameIdentifiers":[{"nameIdentifier":"705149","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"坂下, 哲哉"}],"nameIdentifiers":[{"nameIdentifier":"705150","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小林, 泰彦"}],"nameIdentifiers":[{"nameIdentifier":"705151","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"村上, 健"}],"nameIdentifiers":[{"nameIdentifier":"705152","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鈴木 雅雄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"705153","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"村上 健","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"705154","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"P53-dependent cell-killing effect via bystander effect using carbon-ion microbeams simulating the spot scanning system with pencil beams ","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"P53-dependent cell-killing effect via bystander effect using carbon-ion microbeams simulating the spot scanning system with pencil beams "}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-05-30"},"publish_date":"2015-05-30","publish_status":"0","recid":"71665","relation_version_is_last":true,"title":["P53-dependent cell-killing effect via bystander effect using carbon-ion microbeams simulating the spot scanning system with pencil beams "],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:48:44.596807+00:00"}