{"created":"2023-05-15T14:52:27.036797+00:00","id":71612,"links":{},"metadata":{"_buckets":{"deposit":"9ecba347-e8fe-4fc2-a1e7-3ec383638956"},"_deposit":{"created_by":1,"id":"71612","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"71612"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00071612","sets":["10:28"]},"author_link":["704391","704406","704397","704395","704402","704405","704399","704403","704392","704404","704400","704396","704401","704394","704398","704393"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2013-11-10","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"【目的】アミロイド前駆体蛋白遺伝子改変(APP-Tg)マウスの脳切片を用いて異なるアミロイドβ(Aβ)蓄積 への[11C]PIBの結合強度の相違とその要因を調べる。\n【方法】[11C]PIBのin vitroオートラジオグラフィー(ARG)で、Thioflavin S(Thio-S)添加による[11C]PIBのAβ蓄積への結合阻害実験を実施した。さらにARGの隣接切片を用いてAβのN末端が未修飾のAβN1D(N1D)とN末端が切断修飾されたAβN3pE (N3pE)の抗体染色を行った。\n【結果】100 μMのThio-Sによる結合阻害はAβ斑と一部の血管アミロイドアンギオパチー(CAA)ではみられなかったが、一部のCAAで見られた。隣接切片での抗体染色の結果は結合阻害されないCAA とAβ斑ではN1D、N3pEとも陽性で、Thio-Sで阻害されたCAAではN1D 陽性、N3pE陰性であった。\n【結論】[11C]PIB はN3pE陽性のAβ沈着へはより強い結合を示すことが分かった。このことは、[11C]PIB のAβ沈着への親和性がAβのN末端構造に影響されることを示唆している。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第５３回日本核医学会学術総会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"関, 千江"}],"nameIdentifiers":[{"nameIdentifier":"704391","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小野, 麻衣子"}],"nameIdentifiers":[{"nameIdentifier":"704392","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"季, 斌"}],"nameIdentifiers":[{"nameIdentifier":"704393","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"徳永, 正希"}],"nameIdentifiers":[{"nameIdentifier":"704394","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"丸山, 将浩"}],"nameIdentifiers":[{"nameIdentifier":"704395","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"須原, 哲也"}],"nameIdentifiers":[{"nameIdentifier":"704396","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"樋口, 真人"}],"nameIdentifiers":[{"nameIdentifier":"704397","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"伊藤, 浩"}],"nameIdentifiers":[{"nameIdentifier":"704398","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"関 千江","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"704399","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小野 麻衣子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"704400","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"季 斌","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"704401","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"徳永 正希","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"704402","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"丸山 将浩","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"704403","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"須原 哲也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"704404","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"樋口 真人","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"704405","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"伊藤 浩","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"704406","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"アルツハイマー病モデルマウス脳切片を用いた[11C]PIBの異なるアミロイドβ蓄積病理への結合評価","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"アルツハイマー病モデルマウス脳切片を用いた[11C]PIBの異なるアミロイドβ蓄積病理への結合評価"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-04-20"},"publish_date":"2015-04-20","publish_status":"0","recid":"71612","relation_version_is_last":true,"title":["アルツハイマー病モデルマウス脳切片を用いた[11C]PIBの異なるアミロイドβ蓄積病理への結合評価"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:49:17.768969+00:00"}