{"created":"2023-05-15T14:52:24.645422+00:00","id":71558,"links":{},"metadata":{"_buckets":{"deposit":"753af8cd-ed09-4be3-abf0-7c602b96312c"},"_deposit":{"created_by":1,"id":"71558","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"71558"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00071558","sets":["10:28"]},"author_link":["834617","834618","834610","834605","834616","834619","834622","834611","834613","834608","834609","834620","834606","834607","834621","834615","834614","834612"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2014-11-30","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"【目的】[11C]PIBはアミロイドβ（Aβ）病変を可視化するポジトロン断層撮影（PET）薬剤であるが、いかなるタイプのAβ病変に強く結合するかの詳細は不明である。本研究では、複数系統のアミロイド前駆体蛋白遺伝子改変（APP Tg）マウスのPET所見と脳切片解析データを比較し、各種Aβ病変 への[11C]PIBの結合強度の相違とその要因を調べる。\n【方法】複数系統のAPP Tgマウスに[11C]PIBを投与し、ポジトロン断層撮影（PET）を実施した。次いでこれらのマウスの脳切片を用いて、[11C]PIBのオートラジオグラフィー（ARG）を行った。ARGでは脳実質Aβ斑やアミロイドアンギオパチー（CAA）への [11C]PIBの結合強度を、Thioflavin-S（Thio-S）による 阻害の受けにくさを指標として評価した。さらに隣接切片を用いてAβのN末端が未修飾のAβN1D(N1D)とN末端が切断・修飾されたAβN3pE (N3pE)の抗体染色を行いこれらAβ亜種の含有量と [11C]PIB結合強度との関連を調べた。\n【結果】N1DとN3pEが共に陽性となるAβ斑やCAAを持つAPP Tgマウス系統では、PETで[11C]PIBの集積が多く、ARGで[11C]PIBの結合はThio-Sによる阻害を受けにくかった。N1D陽性・N3pE陰性となるCAAが病変の主体であるAPP Tgマウス系統では、PETで[11C]PIBの集積が少なく、ARGで[11C]PIBの結合はThio-Sによる阻害を受けやすかった。\n【考察】[11C]PIBの親和性がAβのN末端構造に影響され、N3pEの凝集体に強く結合することが示唆された。\n","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第33回日本認知症学会学術集会にてポスター発表を行う。","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"関, 千江"}],"nameIdentifiers":[{"nameIdentifier":"834605","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小野, 麻衣子"}],"nameIdentifiers":[{"nameIdentifier":"834606","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"季, 斌"}],"nameIdentifiers":[{"nameIdentifier":"834607","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"徳永, 正希"}],"nameIdentifiers":[{"nameIdentifier":"834608","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"丸山, 将浩"}],"nameIdentifiers":[{"nameIdentifier":"834609","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"前田, 純"}],"nameIdentifiers":[{"nameIdentifier":"834610","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"須原, 哲也"}],"nameIdentifiers":[{"nameIdentifier":"834611","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"樋口, 真人"}],"nameIdentifiers":[{"nameIdentifier":"834612","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"伊藤, 浩"}],"nameIdentifiers":[{"nameIdentifier":"834613","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Seki, Chie","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"834614","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ono, Maiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"834615","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ji, Bin","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"834616","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tokunaga, Masaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"834617","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Maruyama, Masahiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"834618","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Maeda, Jun","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"834619","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suhara, Tetsuya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"834620","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Higuchi, Makoto","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"834621","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ito, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"834622","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"モデルマウスの画像病理相関解析による [11C]PIBの主要標的病変の同定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"モデルマウスの画像病理相関解析による [11C]PIBの主要標的病変の同定"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-12-03"},"publish_date":"2014-12-03","publish_status":"0","recid":"71558","relation_version_is_last":true,"title":["モデルマウスの画像病理相関解析による [11C]PIBの主要標的病変の同定"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T22:55:32.621499+00:00"}